Activation of human gingival epithelial cells by cell-surface components of black-pigmented bacteria: augmentation of production of interleukin-8, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor and expression of intercellular adhesion molecule 1
| Autor: | T Ogawa, Akiko Uehara, K Matsushita, Shunji Sugawara, Haruhiko Takada, A Sugiyama, R Nakamura, K Iki |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
Keratinocytes Lipopolysaccharides Gingival and periodontal pocket Lipopolysaccharide Neutrophils Intercellular Adhesion Molecule-1 Gingiva Enzyme-Linked Immunosorbent Assay Microbiology Prevotella intermedia chemistry.chemical_compound Granulocyte Colony-Stimulating Factor medicine Humans Periodontal Pocket Interleukin 8 Porphyromonas gingivalis Cells Cultured Glycoproteins Inflammation biology Reverse Transcriptase Polymerase Chain Reaction Interleukin-8 Granulocyte-Macrophage Colony-Stimulating Factor Epithelial Cells General Medicine biology.organism_classification Flow Cytometry Granulocyte macrophage colony-stimulating factor chemistry Fimbriae Bacterial Anaerobic bacteria medicine.drug |
| Zdroj: | Journal of medical microbiology. 51(1) |
| ISSN: | 0022-2615 |
| Popis: | Black-pigmented anaerobic bacteria, such as Porphyromonas gingivalis and Prevotella intermedia, are amongst the predominant bacteria in periodontal pockets and have been implicated in periodontal diseases. To elucidate the roles of gingival keratinocytes, which are the first cells encountered by oral bacteria in periodontal diseases, human gingival keratinocytes in primary culture were stimulated with cell-surface components of P gingivalis and Pr. intermedia. A glycoprotein fraction from Pr. intermedia (PGP) clearly augmented the release of interleukin-8, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor, as determined by enzyme-linked immunosorbent assay. This PGP also induced expression of intercellular adhesion molecule-1 (ICAM-1), as determined by flow cytometry. The augmentation of mRNA expression for these molecules was also confirmed by reverse transcription PCR. In contrast, lipopolysaccharide (LPS) from Pr. intermedia and Escherichia coli was completely inactive in these assays. LPS fraction and purified fimbriae from P gingivalis exhibited weak activities. Cytokine production and ICAM-1 expression by gingival keratinocytes might cause accumulation and activation of neutrophils in the epithelium and, therefore, may be involved in the initiation and development of inflammation in periodontal tissues. |
| Databáze: | OpenAIRE |
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