Prevention of age-induced N(ε)-(carboxymethyl)lysine accumulation in the microvasculature

Autor: Zurab Kakabadze, Koba Kupreishvili, Elisa Meinster, Hans W. N. Niessen, Paul A.J. Krijnen, Ekatarina Berishvili, Hjalmar J. De Graaff, Wessel W. Fuijkschot, Casper G. Schalkwijk, Alexander B.A. Vonk, Yvo M. Smulders, Maaike Houtman, Amber van Broekhoven
Přispěvatelé: Internal medicine, Pathology, ICaR - Circulation and metabolism, Cardio-thoracic surgery, ICaR - Ischemia and repair, Clinical chemistry, ICaR - Heartfailure and pulmonary arterial hypertension, RS: CARIM - R3.01 - Vascular complications of diabetes and the metabolic syndrome, Interne Geneeskunde
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Apolipoprotein E
Aging
Clinical Biochemistry
Coronary Vessels/metabolism
030204 cardiovascular system & hematology
Biochemistry
Superoxide Dismutase/metabolism
0302 clinical medicine
Glycation
hemic and lymphatic diseases
Medicine
Myocardial infarction
Advanced glycation end products
Lysine/analogs & derivatives/metabolism
chemistry.chemical_classification
biology
Brain
General Medicine
docosahexaenoic acid
Coronary Vessels
Survival Rate
Docosahexaenoic acid
Immunohistochemistry
medicine.symptom
microvasculature
medicine.medical_specialty
age-related pathology
Mice
Inbred Strains
Inflammation
Brain/blood supply
Superoxide dismutase
03 medical and health sciences
N(epsilon)-(carboxymethyl)lysine (CML)
Apolipoproteins E
Internal medicine
Animals
Superoxide Dismutase
business.industry
Lysine
Fatty acid
Apolipoproteins E/deficiency
medicine.disease
Aging/metabolism
030104 developmental biology
Endocrinology
chemistry
Microvessels
Microvessels/metabolism
biology.protein
Endothelium
Vascular
business
Endothelium
Vascular/metabolism
Zdroj: European Journal of Clinical Investigation, 46(4), 334-341
Fuijkschot, W W, de Graaff, H J, Berishvili, E, Kakabadze, Z, Kupreishvili, K, Meinster, E, Houtman, M, van Broekhoven, A, Schalkwijk, C G, Vonk, A B A, Krijnen, P A J, Smulders, Y M & Niessen, H W N 2016, ' Prevention of age-induced N(ε)-(carboxymethyl)lysine accumulation in the microvasculature ', European Journal of Clinical Investigation, vol. 46, no. 4, pp. 334-341 . https://doi.org/10.1111/eci.12599
European Journal of Clinical Investigation, Vol. 46, No 4 (2016) pp. 334-341
European Journal of Clinical Investigation, 46(4), 334-341. Wiley
ISSN: 0014-2972
Popis: OBJECTIVE: N(ε)-(carboxymethyl)lysine (CML) is one of the major advanced glycation end products in both diabetics and nondiabetics. CML depositions in the microvasculature have recently been linked to the aetiology of acute myocardial infarction and cognitive impairment in Alzheimer's disease, possibly related to local enhancement of inflammation and oxidative processes. We hypothesized that CML deposition in the microvasculature of the heart and brain is age-induced and that it could be inhibited by a diet intervention with docosahexaenoic acid (DHA), an omega-3 fatty acid known for its anti-inflammatory and antioxidative actions.MATERIALS AND METHODS: ApoE(-/-) mice (n = 50) were fed a Western diet and were sacrificed after 40, 70 and 90 weeks. Part of these mice (n = 20) were fed a Western diet enriched with DHA from 40 weeks on. CML in cardiac and cerebral microvessels was quantified using immunohistochemistry.RESULTS: Cardiac microvascular depositions of CML significantly increased with an immunohistochemical score of 11·85 [5·92-14·60] at 40 weeks, to 33·17 [17·60-47·15] at 70 weeks (P = 0·005). At the same time points, cerebral microvascular CML increased from 6·45; [4·78-7·30] to 12·99; [9·85-20·122] (P = 0·003). DHA decreased CML in the intramyocardial vasculature at both 70 and 90 weeks, significant at 70 weeks [33·17; (17·60-47·15) vs. 14·73; (4·44-28·16) P = 0·037]. No such effects were found in the brain.CONCLUSIONS: Accumulation of N(ε)-(carboxymethyl)lysine in the cerebral and cardiac microvasculature is age-induced and is prevented by DHA in the intramyocardial vessels of ApoE(-/-) mice.
Databáze: OpenAIRE
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