Immunocytochemical demonstration of neuropeptides in the central nervous system of the roundworm, Ascaris suum (Nematoda: Ascaroidea)
| Autor: | D. Smart, Ian Fairweather, Colin F. Johnston, D W Halton, D.J.A. Brownlee, Christopher Shaw |
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| Rok vydání: | 1993 |
| Předmět: |
Central Nervous System
Invertebrate Hormones Central nervous system Substance P Biology Chromogranins medicine Animals Pancreatic polypeptide Neuropeptide Y FMRFamide Ascaris suum Neurotransmitter Agents Plexus Neuropeptides Anatomy biology.organism_classification Immunohistochemistry Infectious Diseases medicine.anatomical_structure Peptide YY Peripheral nervous system Chromogranin A Animal Science and Zoology Parasitology Cholecystokinin Atrial Natriuretic Factor Immunostaining |
| Zdroj: | Parasitology. 106:305-316 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182000075132 |
| Popis: | The localisation and distribution of neuropeptides in the peripheral nervous system of the pig roundwormAscaris suum have been determined by an indirect immunofluoresence technique in conjunction with confocal microscopy. Of the 31 antisera tested, immunostaining was obtained only with antisera to peptide YY (PYY), pancreatic polypeptide (PP) and FMRFamide. Immunostaining for PYY and FMRFamide was evident in the amphidial and papillary ganglia associated with the anterior nerve ring and in the nerves from these ganglia that terminated in sensory receptors within the buccal lips of the parasite. The only peptide immunoreactivity (IR) observed in the reproductive system of either sex was that evident in the nerve supply to the distal region of the vagina in the female worm. It took the form of a well-developed plexus of parallel nerve fibres, cross-connectives and looped commissures. The nerve net diminished in the more proximal region of the vagina. PP-IR was less intense than that for PYY and FMRFamide and was more restricted in distribution, being confined to a small number of nerve fibres in the nerve supply to the vagina; it did not occur in the nerves supplying the anterior sensory receptors. The possible roles of neuropeptides in the sensory and reproductive biology of nematodes are discussed. |
| Databáze: | OpenAIRE |
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