A low-cost polyacrylamide gel scanning densitometer

Autor: David K. Shortess
Rok vydání: 1979
Předmět:
Zdroj: Analytical biochemistry. 100(1)
ISSN: 0003-2697
Popis: A simple, low-cost, home-built acrylamide gel scanning densitometer is described. The instrument was designed to provide gel scans for the purpose of comparing the relative rates of mobility of bands. It does not include provisions for large volume, integration of peaks, or micrometer resolution. The key to the simplicity of the device was through the use of a rotary rather than a transverse gel carriage. A turntable was attached directly to the shaft of a synchronous clock motor with a slow rate of rotation. A curved gel holder, placed on the turntable, carries the gel through the light beam. A commercial strip chart recorder is used with the scanner.
Databáze: OpenAIRE
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