Quartz crystal microbalance-based biosensor for the detection of α-thalassemia 1 (SEA deletion)
| Autor: | Wanida Ittarat, Tiparat Potipitak, Chamras Promptmas, Sirinart Chomean |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Clinical Biochemistry Biotin Biosensing Techniques Polymerase Chain Reaction chemistry.chemical_compound alpha-Thalassemia hemic and lymphatic diseases Humans Biotinylation Electrodes DNA Primers Gel electrophoresis biology Chemistry DNA–DNA hybridization Hybridization probe Biochemistry (medical) Nucleic Acid Hybridization Reproducibility of Results General Medicine Quartz crystal microbalance DNA Avidin Molecular biology biology.protein Quartz Crystal Microbalance Techniques Gold Oligonucleotide Probes Biosensor |
| Zdroj: | Clinical chemistry and laboratory medicine. 48(9) |
| ISSN: | 1437-4331 |
| Popis: | Background: DNA piezoelectric biosensors have become a promising tool in molecular medicine since they do not require any label or staining. Here, a DNA piezoelectric biosensor based on a quartz crystal microbalance (QCM) was created to identify abnormal genes causing α-thalassemia 1 (SEA deletion). Methods: The functionalized gold electrode of the quartz crystal was coated with avidin and the biotinylated DNA probe was attached. The target gene causing α-thalassemia 1 was amplified and hybridized with the immobilized probe. DNA hybridization was indicated by changes in the quartz resonance frequencies. Diagnostic ability of the new α-thalassemia 1 biosensor was validated using both known and unknown blood samples. Specificity was tested using samples of β-thalassemia and α-thalassemia 2. Stability of the sensor was also evaluated. Results: The new biosensor could clearly identify α-thalassemia 1 (SEA deletion), both carrier and disease states, from the normal genotype. Identification accuracy was compatible to the standard gel electrophoresis. It was specific only to α-thalassemia 1 since no cross reaction was found with β-thalassemia and α-thalassemia 2. The sensor could be kept at room temperature up to 6 months with consistent identification accuracy. Conclusions: The label free QCM based biosensor was successfully developed to diagnose an abnormal human globin gene causing α-thalassemia 1 (SEA deletion). Its accuracy, specificity and sensitivity were comparable to the standard method. Its stable diagnostic potency up to 6 months implied its field application in thalassemic control program. Clin Chem Lab Med 2010;48:1247–54. |
| Databáze: | OpenAIRE |
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