Diversity of repetitive sequences within compact genomes of Phaseolus L. beans and allied genera Cajanus L. and Vigna Savi
| Autor: | Andrea Pedrosa-Harand, Magdalena Vaio, Ana Christina Brasileiro-Vidal, Tiago Ribeiro, Emanuelle Varão Vasconcelos, Karla G. B. dos Santos |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
DNA Plant Retroelements Retrotransposon 01 natural sciences Genome Chromosomes Plant Vigna 03 medical and health sciences Cajanus Genetic Heterogeneity Genetics Data Mining Copy-number variation Phaseoleae Genome size In Situ Hybridization Fluorescence Phylogeny 030304 developmental biology Repetitive Sequences Nucleic Acid Phaseolus 0303 health sciences biology food and beverages Computational Biology Genetic Variation Genomics biology.organism_classification Evolutionary biology Tandem Repeat Sequences Genome Plant 010606 plant biology & botany |
| Zdroj: | Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology. 28(2) |
| ISSN: | 1573-6849 |
| Popis: | Repetitive sequences are ubiquitous and fast-evolving elements responsible for size variation and large-scale organization of plant genomes. Within tribe Phaseoleae (Fabaceae), some genera, such as Phaseolus, Vigna, and Cajanus, show small genome and mostly stable chromosome number. Here, we applied a combined computational and cytological approach to study the organization and diversification of repetitive elements in some species of these genera. Sequences were classified in terms of type and repetitiveness and the most abundant were mapped to chromosomes. We identified long terminal repeat (LTR) retrotransposons, especially Ogre and Chromovirus elements, making up most of genomes, other than P. acutifolius and Vigna species. Satellite DNAs (SatDNAs) were less representative, but highly diverse among species, showing a clear phylogenetic relationship. In situ localization revealed preferential location at pericentromeres and centromeres for both types of sequences, suggesting a heterogeneous composition, especially for centromeres. Few elements showed subterminal accumulation. Copy number variation among chromosomes within and among species was observed for all nine identified SatDNAs. Altogether, our data pointed two main elements (Ty3/Gypsy retrotransponsons and SatDNAs) to the diversification on the repetitive landscape in Phaseoleae, with a typical set of repeats in each species. The high turnover of these sequences, however, did not affect total genome size. |
| Databáze: | OpenAIRE |
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