Simultaneous determination of eight metabolites of organophosphate and pyrethroid pesticides in urine
| Autor: | Meng Y Huang, Na Wang, Xin Y Guo, Li S Sun, Ying Wang, Wei L Xu |
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| Rok vydání: | 2016 |
| Předmět: |
Analyte
Internal standard Calibration curve Liquid-Liquid Extraction 010501 environmental sciences Mass spectrometry 01 natural sciences Gas Chromatography-Mass Spectrometry Organophosphorus Compounds Limit of Detection Tandem Mass Spectrometry Pyrethrins Humans Solid phase extraction Pesticides 0105 earth and related environmental sciences Chromatography Gas Chromatography/Tandem Mass Spectrometry Chemistry Solid Phase Extraction 010401 analytical chemistry Selected reaction monitoring Extraction (chemistry) Reproducibility of Results Environmental Exposure General Medicine Pollution Organophosphates 0104 chemical sciences Calibration Food Science |
| Zdroj: | Journal of Environmental Science and Health, Part B. 52:1-9 |
| ISSN: | 1532-4109 0360-1234 |
| DOI: | 10.1080/03601234.2016.1224695 |
| Popis: | A simultaneous method for quantifying eight metabolites of organophosphate pesticides and pyrethroid pesticides in urine samples has been established. The analytes were extracted using liquid–liquid extraction coupled with WCX solid phase extraction (SPE) cartridges. Eight metabolites were chemically derivatized before analysis using gas chromatography–tandem mass spectrometry (GC–MS–MS). The separation was performed on a HP-5MS capillary column (30 m × 0.25 mm × 0.25 µm) with temperature programming. The detection was performed under electro-spray ionization (ESI) in multiple reaction monitoring (MRM) mode. An internal standard method was used. The extraction solvent, types of SPE cartridges and eluents were optimized by comparing the sample recoveries under different conditions. The results showed that the calibration curves of the five organophosphorus pesticides metabolites were linear in the range of 0.2–200 μg/L (r2 ≥ 0.992) and that of the three pyrethroid pesticides metabolites were linear... |
| Databáze: | OpenAIRE |
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