Continuous monitoring of extracellular lactate concentration by microdialysis lactography for the study of rat muscle metabolism in vivo
| Autor: | H Plijtergroendijk, Folkert Postema, Jakob Korf, Jaep de Boer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Microdialysis Physiology FLOW Clinical Biochemistry Fluorescence spectrometry Iodoacetates EXERCISE PRESSURE LACTATE PROBES Gastrocnemius muscle chemistry.chemical_compound Tibialis anterior muscle Physiology (medical) Internal medicine CONTRACTION DIALYSIS medicine Extracellular Animals Lactic Acid Monitoring Physiologic LACTIC-ACID Chemistry Muscles EXTRACELLULAR Osmolar Concentration Skeletal muscle Rats Inbred Strains Metabolism MUSCLE Lactic acid Iodoacetic Acid Rats ISCHEMIA Endocrinology medicine.anatomical_structure Biochemistry BRAIN DIALYSIS Lactates SKELETAL-MUSCLE Extracellular Space Glycolysis |
| Zdroj: | Pflugers archiv-European journal of physiology, 419(1), 1-6. SPRINGER |
| ISSN: | 0031-6768 |
| DOI: | 10.1007/bf00373739 |
| Popis: | A method is described for the measurement and on-line monitoring of muscular extracellular lactate concentration in both anaesthetized and freely moving rats. This method is based on microdialysis sampling and lactic dehydrogenase-catalysed nicotinamide adenine dinucleotide, reduced (NADH)-fluorescence detection techniques. In vivo calibration revealed a resting extracellular lactate concentration of 1.92 +/- 0.13 mmol/l (+/- SEM) in the gastrocnemius muscle of adult male Wistar rats (n = 6), while the average whole-blood lactate level was 0.76 +/- 0.12 mmol/l (+/- SEM). This measured extracellular lactate concentration was 1.73-times higher than that deduced from the arterial lactate concentration. Blocking glycolysis with iodoacetate reduced the extracellular lactate concentration to 52 + 6% (+/- SEM, n = 4) of the resting level. The extracellular lactate concentration in rat gastrocnemius muscle had increased to significantly (P less-than-or-equal-to 0.05) different levels, 2.4 +/- 0.03 (+/- SEM) or 4.0 +/- 0.55 (+/- SEM) times the control value, 1 h after aortic clamping (n = 3) or cardiac arrest (n = 3), respectively. Stimulation of the sciatic nerve induced elevations of the extracellular lactate concentration in the tibialis anterior muscle which were linearly related to the recorded isometric force-time integral. We also monitored on-line the changes in extracellular lactate concentration in the tibialis anterior muscle of a swimming rat. Our results indicate that microdialysis lactate reflects also intracellular metabolism. Lactography may be a useful alternative to biopsies and nuclear magnetic resonance spectroscopy in clinical medicine and physiology for the monitoring of metabolism in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink