Characterization of human chromosomal DNA sequences which replicate autonomously in Saccharomyces cerevisiae
| Autor: | A J Kingsman, J. F. Montiel, C. J. Norbury, Mick F. Tuite, Susan M. Kingsman, John S. Mills, Melanie J. Dobson |
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| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
Genetics
DNA Replication Base Sequence Autonomously replicating sequence Nucleic acid sequence DNA replication Nucleic Acid Hybridization DNA DNA Restriction Enzymes Saccharomyces cerevisiae Biology Restriction fragment Conserved sequence Restriction map Plasmid Consensus sequence biology.protein Escherichia coli Chromosomes Human Humans Chromosome Deletion Plasmids |
| Zdroj: | Nucleic acids research. 12(2) |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | We have characterised two restriction fragments, isolated from a "shotgun" collection of human DNA, which function as autonomously replicating sequences (ARSs) in Saccharomyces cerevisiae. Functional domains of these fragments have been defined by subcloning and exonuclease (BAL 31) deletion analysis. Both fragments contain two spatially distinct domains. One is essential for high frequency transformation and is termed the Replication Sequence (RS) domain, the other, termed the Replication Enhancer (RE) domain, has no inherent replication competence but is essential for ensuring maximum function of the RS domain. The nucleotide sequence of these domains reveals several conserved sequences one of which is strikingly similar to the yeast ARS consensus sequence. |
| Databáze: | OpenAIRE |
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