Functional genomics of histone modification and non-histone chromosomal proteins using the polytene chromosomes of Drosophila
| Autor: | Joel C. Eissenberg |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chromosomal Proteins
Non-Histone Immunofluorescence Chromosomes Salivary Glands General Biochemistry Genetics and Molecular Biology Histones Non histone chromosomal medicine Animals Drosophila Proteins Drosophila (subgenus) Molecular Biology Cell Nucleus Genetics Polytene chromosome biology medicine.diagnostic_test Chromosome Mapping Genomics biology.organism_classification Histone biology.protein Drosophila Functional genomics Chromatin immunoprecipitation Function (biology) |
| Zdroj: | Methods. 40:360-364 |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/j.ymeth.2006.09.001 |
| Popis: | Knowing the genomic distribution of chromosomal proteins and of histone modifications provides essential insight into function. The giant polytene chromosomes of the Drosophila larval salivary glands provide a high-resolution genomic map with a resolution exceeded only by chromatin immunoprecipitation. Immunofluorescence localization of chromosomal proteins and specific post-translational modifications of histones is a simple and rapid tool for the functional genomics of chromosomal proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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