Cadherin-based intercellular adhesions organize epithelial cell–matrix traction forces
| Autor: | M. Cristina Marchetti, Shiladitya Banerjee, Kathryn A. Rosowski, Yonglu Che, Eric R. Dufresne, Stephen F. Revilla, Jill M. Goldstein, Carien M. Niessen, Aaron F. Mertz, Valerie Horsley |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Keratinocytes
Role of cell adhesions in neural development FOS: Physical sciences Condensed Matter - Soft Condensed Matter Biology Cell junction Traction force microscopy Mechanotransduction Cellular Models Biological Biophysical Phenomena Adherens junction Extracellular matrix 03 medical and health sciences Gene Knockout Techniques Mice 0302 clinical medicine Cell Behavior (q-bio.CB) Cell Adhesion Animals Physics - Biological Physics Mechanotransduction RNA Small Interfering Cell adhesion Cells Cultured 030304 developmental biology 0303 health sciences Multidisciplinary Cadherin Cadherins 3. Good health Cell biology Culture Media Intercellular Junctions Biological Physics (physics.bio-ph) FOS: Biological sciences Gene Knockdown Techniques Physical Sciences Soft Condensed Matter (cond-mat.soft) Quantitative Biology - Cell Behavior Calcium 030217 neurology & neurosurgery |
| Popis: | Cell--cell and cell-matrix adhesions play essential roles in the function of tissues. There is growing evidence for the importance of crosstalk between these two adhesion types, yet little is known about the impact of these interactions on the mechanical coupling of cells to the extracellular-matrix (ECM). Here, we combine experiment and theory to reveal how intercellular adhesions modulate forces transmitted to the ECM. In the absence of cadherin-based adhesions, primary mouse keratinocytes within a colony appear to act independently, with significant traction forces extending throughout the colony. In contrast, with strong cadherin-based adhesions, keratinocytes in a cohesive colony localize traction forces to the colony periphery. Through genetic or antibody-mediated loss of cadherin expression or function, we show that cadherin-based adhesions are essential for this mechanical cooperativity. A minimal physical model in which cell--cell adhesions modulate the physical cohesion between contractile cells is sufficient to recreate the spatial rearrangement of traction forces observed experimentally with varying strength of cadherin-based adhesions. This work defines the importance of cadherin-based cell--cell adhesions in coordinating mechanical activity of epithelial cells and has implications for the mechanical regulation of epithelial tissues during development, homeostasis, and disease. 10 pages, 6 figures |
| Databáze: | OpenAIRE |
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