Breaking the resistance of Escherichia coli: Antimicrobial activity of Berberis lycium Royle
| Autor: | Tauseef Ahmad Malik, Mudasir A. Tantry, P.R. Hussain, Shazia Ahad, R.K. Johri, Azra N. Kamili, M. Z. Chishti |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antifungal Agents Berberis Microbial Sensitivity Tests medicine.disease_cause 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Berberine Anti-Infective Agents Drug Resistance Bacterial medicine Escherichia coli Agar diffusion test Candida albicans Chromatography High Pressure Liquid biology Traditional medicine Plant Extracts Fungi biology.organism_classification Antimicrobial Corpus albicans Anti-Bacterial Agents 030104 developmental biology Infectious Diseases chemistry visual_art visual_art.visual_art_medium Bark Lycium 010606 plant biology & botany |
| Zdroj: | Microbial pathogenesis. 102 |
| ISSN: | 1096-1208 |
| Popis: | The antimicrobial activity of root bark of Berberis lycium and its principal component berberine was tested against a panel of microbial strains using agar well diffusion test and further analyzed using micro-broth dilution method. Preliminary analysis, on the basis of zone of Inhibition (ZOI) showed that the methanolic extract of B. lycium was highly effective against Escherichia coli (ZOI 41 ± 1 mm). Among the bacterial strains E. coli was found to be most susceptible and among fungi Candida albicans was the most susceptible for berberine as well as the crude methanolic extract of the plant. Methanolic extract of the plant was more effective for E. coli (MIC 1.7 ± 1.18; MBC 2.4 ± 1.18) than berberine (MIC 3.5 ± 0.57) (p < 0.05), whereas berberine was more effective than crude extracts for C. albicans. In addition, E. coli showed the development of resistant colonies after 72 h when tested with berberine but the development of such colonies was not observed with the methanolic extract of the plant. This could be due to the presence of resistance breaking molecules in the crude methanolic extract of B. lycium. Also the MIC index of crude methanolic extract was 1.39 for E. coli, which showed the mode of action to be bactericidal. HPLC analysis revealed the presence of berberine at highest concentration in methanolic extract of the plant, followed by aqueous extract. Potentiation of this berberine by resistance breaking molecules in the crude extract could be a possible explanation for its strong effectiveness. |
| Databáze: | OpenAIRE |
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