SFRS7-Mediated Splicing of Tau Exon 10 Is Directly Regulated by STOX1A in Glial Cells
| Autor: | Cees B.M. Oudejans, Rossa W.K. Chiu, Marie van Dijk, Yama W. L. Zheng, Eric Z. Chen, Dennis R. Hölzel, Daan van Abel, Shushant Jain, Fiona M.F. Lun, Hao Sun, Y.M. Dennis Lo |
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| Přispěvatelé: | Clinical chemistry, Human genetics, NCA - Neurodegeneration, ICaR - Ischemia and repair |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Nucleocytoplasmic Transport Proteins
Science RNA Splicing Tau protein DNA transcription RNA-binding protein tau Proteins Biology Transfection Gene Splicing Exon Splicing factor Cell Line Tumor Gene expression Neurobiology of Disease and Regeneration Genetics Humans RNA Messenger Nuclear protein Promoter Regions Genetic Messenger RNA Multidisciplinary Serine-Arginine Splicing Factors High-Throughput Nucleotide Sequencing Nuclear Proteins RNA-Binding Proteins Reproducibility of Results Neurodegenerative Diseases DNA Exons Molecular biology Neurology RNA splicing biology.protein Medicine Carrier Proteins Neuroglia Research Article Neuroscience Protein Binding |
| Zdroj: | PLoS ONE PLoS ONE, 6(7):e21994. Public Library of Science van Abel, D, Holzel, D R, Jain, S, Lun, F M F, Zheng, Y M W L, Chen, E Z, Sun, H, Chiu, R W K, Lo, Y M D, van Dijk, M & Oudejans, C B M 2011, ' SFRS7-Mediated Splicing of Tau Exon 10 Is Directly Regulated by STOX1A in Glial Cells ', PLoS ONE, vol. 6, no. 7, e21994 . https://doi.org/10.1371/journal.pone.0021994 PLoS ONE, Vol 6, Iss 7, p e21994 (2011) |
| ISSN: | 1932-6203 |
| Popis: | BackgroundIn this study, we performed a genome-wide search for effector genes bound by STOX1A, a winged helix transcription factor recently demonstrated to be involved in late onset Alzheimer's disease and affecting the amyloid processing pathway.Methodology/principal findingsOur results show that out of 218 genes bound by STOX1A as identified by chromatin-immunoprecipitation followed by sequencing (ChIP-Seq), the serine/arginine-rich splicing factor 7 (SFRS7) was found to be induced, both at the mRNA and protein levels, by STOX1A after stable transfection in glial cells. The increase in SFRS7 was followed by an increase in the 4R/3R ratios of the microtubule-associated protein tau (MAPT) by differential exon 10 splicing. Secondly, STOX1A also induced expression of total tau both at the mRNA and protein levels. Upregulation of total tau expression (SFRS7-independent) and tau exon 10 splicing (SFRS7-dependent), as shown in this study to be both affected by STOX1A, is known to have implications in neurodegeneration.ConclusionsOur data further supports the functional importance and central role of STOX1A in neurodegeneration. |
| Databáze: | OpenAIRE |
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