Comparative study on the antituberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiaoHe extract and JieHeWan
| Autor: | Yan Liang, Jie Mi, Yue Zheng, Lan Wang, Zai-Xing Jia, Jie Wang, Yu-Mei Liang, Xueqiong Wu, Hong Lei, Li-Yao Duan, Jun Liu, Yong Xue, Wenping Gong |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Medicine (General) Tuberculosis 030106 microbiology Antitubercular Agents Pharmacology Lesion Mycobacterium tuberculosis Mice 03 medical and health sciences Immune system Traditional Chinese medicine R5-920 medicine Animals Medicine Chinese Traditional Lung JieHeWan Mice Inbred BALB C biology business.industry Research ELISPOT Therapeutic effect General Medicine biology.organism_classification medicine.disease Disease Models Animal 030104 developmental biology medicine.anatomical_structure Military Science NiuBeiXiaoHe extract Female Histopathology medicine.symptom business Drugs Chinese Herbal |
| Zdroj: | Military Medical Research, Vol 8, Iss 1, Pp 1-22 (2021) Military Medical Research |
| ISSN: | 2054-9369 |
| Popis: | Background The traditional Chinese medicine NiuBeiXiaoHe (NBXH) extract and Chinese medicine preparation JieHeWan (JHW) exhibit anti-tuberculosis effects. The anti- tuberculosis effect of NBXH was compared with that of JHW to elucidate the mechanism of action of NBXH. Methods BALB/c mice aged 6-8 weeks were randomly divided into a normal control group, Tuberculosis (TB) model group, JHW treatment group, and NBXH treatment group. After 3 and 13 weeks of treatment, the therapeutic effect in each group was evaluated by comparing lung histopathology, lung and liver colony counts, the number of spots representing effector T cells secreting IFN-γ in an ELISPOT, and the levels of Th1, Th2, and Th17 cytokines, which were measured by a cytometric bead array (CBA). Mouse RNA samples were subjected to transcriptome sequencing. Results After 13 weeks of treatment, the mean histopathological lesion area of the NBXH group was significantly smaller than that of the TB model group (P P P Mycobacterium tuberculosis (M. tuberculosis) infection. According to GO and KEGG analyses, the changes in biological process (BP), cell composition (CC) and molecular function (MF) terms and in signaling pathways caused by NBXH and JHW treatment were not completely consistent, but they were mainly related to the immune response and inflammatory response in the mouse TB model. Conclusions NBXH had therapeutic effects similar to those of JHW in improving lung histopathology, reducing lung colony counts, and regulating the levels of cytokines. NBXH restored significant changes in gene expression and repaired cell damage caused by M. tuberculosis infection by regulating immune-related pathways, which clarified the mechanism of action of NBXH. |
| Databáze: | OpenAIRE |
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