Time-series proteomic study of the response of HK-2 cells to hyperglycemic, hypoxic diabetic-like milieu

Autor:	María Luisa Marina, Alberto Valdés, María Castro-Puyana, Coral García-Pastor, Francisco Javier Lucio-Cazaña
Přispěvatelé:	Universidad de Alcalá. Departamento de Biología de Sistemas, Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química
Rok vydání:	2020
Předmět:	0301 basic medicine Proteomics Time Factors Proteome Pulmonology Cell Protein Expression 030204 cardiovascular system & hematology Biochemistry Kidney Tubules Proximal 0302 clinical medicine Endocrinology Tandem Mass Spectrometry Medicine and Health Sciences Glycolysis Diabetic Nephropathies Protein Interaction Maps Hypoxia Chromatography High Pressure Liquid Cytoskeleton Multidisciplinary Chemistry Organic Compounds Monosaccharides Química Cell Hypoxia medicine.anatomical_structure Physical Sciences Medicine medicine.symptom Cellular Structures and Organelles Research Article medicine.medical_specialty Epithelial-Mesenchymal Transition Endocrine Disorders Science Quantitative proteomics Carbohydrates Carbohydrate metabolism Research and Analysis Methods Cell Line 03 medical and health sciences Internal medicine Diabetes mellitus Medical Hypoxia medicine Diabetes Mellitus Gene Expression and Vector Techniques Humans Molecular Biology Techniques Molecular Biology Molecular Biology Assays and Analysis Techniques Organic Chemistry Chemical Compounds Biology and Life Sciences Epithelial Cells Cell Biology Hypoxia (medical) medicine.disease Fibrosis In vitro 030104 developmental biology Glucose Cell culture Metabolic Disorders Developmental Biology
Zdroj:	PLoS ONE e_Buah Biblioteca Digital Universidad de Alcalá instname PLoS ONE, Vol 15, Iss 6, p e0235118 (2020)
ISSN:	1932-6203
Popis:	During diabetes, renal proximal tubular cells (PTC) are exposed to a combination of high glucose and hypoxic conditions, which plays a relevant role in the development of diabetic kidney disease (DKD). In this work, a time-series proteomic study was performed to analyse the effect of a diabetic-like microenvironment induced changes on HK-2 cells, a human cell line derived from normal proximal tubular epithelial cells. Cells simultaneously exposed to high glucose (25 mM) and hypoxia (1% O-2) were compared to cells in control conditions for up to 48 h. Diabetic conditions increased the percentage of death cells after 24 and 48 h, but no differences in the protein/cell ratio were found. The relative protein quantification using dimethyl-labeling and UHPLC-MS/MS analysis allowed the identification of 317, 296 and 259 proteins at 5, 24 and 48 h, respectively. The combination of statistical and time expression profile analyses indicated an increased expression of proteins involved in glycolysis, and a decrease of cytoskeletal-related proteins. The exposure of HK-2 cells to high glucose and hypoxia reproduces some of the effects of diabetes on PTC and, with the limitations inherent toin vitrostudies, propose new mechanisms and targets to be considered in the management of DKD.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54275e805166dc0177daacb1585dc5a1 https://pubmed.ncbi.nlm.nih.gov/32579601 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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