Biological Effects of the Novel Mulberry Surface Characterized by Micro/Nanopores and Plasma-Based Graphene Oxide Deposition on Titanium
| Autor: | Hyun-Seung Kim, Hoonsung Cho, Khurshed Alam, Woohyung Jang, Min-Kyung Ji, Hyun-Pil Lim, Hee-Seon Kim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Biocompatibility Surface Properties Biophysics Oxide Pharmaceutical Science chemistry.chemical_element Bioengineering Osseointegration law.invention Biomaterials Nanopores chemistry.chemical_compound International Journal of Nanomedicine law Drug Discovery Original Research Titanium Osteoblasts Graphene Organic Chemistry anodic oxidation General Medicine Adhesion nitriding Nanopore chemistry Chemical engineering biofilm formation Graphite Morus atmospheric plasma Nitriding |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s311872 |
| Popis: | Hee-Seon Kim1 *, Min-Kyung Ji2 *, Woo-Hyung Jang1 *, Khurshed Alam,3 Hyun-Seung Kim,4 Hoon-Sung Cho,3 Hyun-Pil Lim1 1Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea; 2Optoelectronics Convergence Research Center, Chonnam National University, Gwangju, 61186, Republic of Korea; 3Department of Materials Science and Engineering, Chonnam National University, Gwangju, 61186, Republic of Korea; 4Department of Division of New Projects, KJ Meditech Co, Ltd, Gwangju, 61009, Republic of Korea*These authors contributed equally to this workCorrespondence: Hyun-Pil LimDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, 61186, Republic of KoreaTel +82-10-2645-7528Fax +82-62-530-5577Email mcnihil@jnu.ac.krHoon-Sung ChoDepartment of Materials Science and Engineering, Chonnam National University, Gwangju, 61186, Republic of KoreaTel +82-10-8545-2816Fax +82-62-530-1717Email cho.hoonsung@jnu.ac.krPurpose: This paper presents a technique for developing a novel surface for dental implants using a combination of nitriding and anodic oxidation, followed by the deposition of graphene oxide using atmospheric plasma. The effects of various surface treatments on bacterial adhesion and osteoblast activation were also evaluated.Methods: CP titanium (control) was processed into disk-shaped specimens. Nitriding was conducted using vacuum nitriding, followed by anodic oxidation, which was performed in an electrolyte using a DC power supply, to form the novel âmulberry surface.â Graphene oxide deposition was performed using atmospheric plasma with an inflow of carbon sources. After analyzing the sample surfaces, antibacterial activity was evaluated using Streptococcus mutans and Porphyromonas gingivalis bacteria. The viability, adhesion, proliferation, and differentiation of osteoblasts were also assessed. Analysis of variance (ANOVA) with Tukeyâs post-hoc test was used to calculate statistical differences.Results: We observed that the mulberry surface was formed on samples treated with nitriding and anodic oxidation, and these samples exhibited more effective antibacterial activity than the control. We also found that the samples with additional graphene oxide deposition exhibited better biocompatibility, which was validated by osteoblast adhesion, proliferation, and differentiation.Conclusion: The development of the mulberry surface along with graphene oxide deposition inhibits bacterial adhesion to the implant and enhances the adhesion, proliferation, and differentiation of osteoblasts. These results indicate that the mulberry surface and graphene oxide deposition together can inhibit peri-implantitis and promote osseointegration.Keywords: nitriding, anodic oxidation, atmospheric plasma, biofilm formation, osteoblasts |
| Databáze: | OpenAIRE |
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