Effects of Inflammatory Mediators on the Responsiveness of Isolated Human Airways to Methacholine
| Autor: | I. L. Bonta, J. C. De Jongste, Theo Stijnen, R. C. Jongejan, R. C. Raatgeep, K. F. Kerrebijn |
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| Rok vydání: | 1990 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine medicine.medical_specialty Prostaglandin Bronchi Inflammation In Vitro Techniques Dinoprost chemistry.chemical_compound Thromboxane A2 Internal medicine Humans Medicine Respiratory system Methacholine Chloride Aged Dose-Response Relationship Drug Leukotriene C4 Prostaglandin D2 business.industry Middle Aged respiratory system Prostaglandin Endoperoxides Synthetic Endocrinology chemistry Female SRS-A lipids (amino acids peptides and proteins) Methacholine medicine.symptom business Histamine Muscle Contraction medicine.drug |
| Zdroj: | American Review of Respiratory Disease. 142:1129-1132 |
| ISSN: | 0003-0805 |
| DOI: | 10.1164/ajrccm/142.5.1129 |
| Popis: | Several studies have suggested that in asthmatics the quantities of inflammatory mediators such as histamine, thromboxane A2 (TxA2), prostaglandin D2 (PGD2), prostaglandin F2 alpha (PGF2 alpha), and leukotriene C4 (LTC4) that are present in the airway lumen are related to the degree of bronchial responsiveness to inhaled methacholine (MCh). Therefore, we studied the effect of these mediators on the cholinergic responsiveness of isolated human airway segments. Lung tissue collected at thoracotomy from 30 patients was studied. Dose-response curves to MCh were obtained from bronchial segments before, during, and after incubation with either a subthreshold or a threshold concentration of histamine (10(-10) or 10(-8) M), the stable TxA2 analogue U46619 (10(-11) or 10(-9) M), PGD2 (5 x 10(-9) or 5 x 10(-7) M), PGF2 alpha (10(-9) or 10(-7) M), or LTC4 (10(-11) or 10(-9) M). With the exception of LTC4, the presence of any of these mediators at either concentration increased the sensitivity to MCh by a factor of 1.1 to 2 (p less than 0.05, ANOVA). This increase did not depend on the dose of the mediator (p greater than 0.05, ANOVA). These data indicate that mediator-induced muscle hypersensitivity can explain a small part of the leftward shift of the dose-response curve to inhaled MCh as observed in asthma. |
| Databáze: | OpenAIRE |
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