Efectos de la genisteína en los niveles del Ca2+ citosólico en células musculares de arteria umbilical humana
| Autor: | Franco Tanzi, Silvia Salemme, María Cristina Añón, Verónica Milesi, Alejandro Rebolledo, Laura Rimorini, A. Spinillo, Francisco Speroni, R. Roldán-Palomo |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Patch-Clamp Techniques Physiology Biología Genistein Biology Biochemistry Muscle Smooth Vascular Umbilical Arteries chemistry.chemical_compound Pregnancy Placenta Internal medicine medicine Extracellular Humans Patch clamp Human vascular smooth muscle Voltage-dependent calcium channel General Medicine Isoflavones Calcium channels Sarcoplasmic Reticulum Umbilical artery Endocrinology medicine.anatomical_structure chemistry Calcium Female Phytoestrogens Calcium Channels Intracellular |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| ISSN: | 1877-8755 1138-7548 |
| DOI: | 10.1007/bf03179062 |
| Popis: | Isoflavones are a group of natural phytoestrogens including the compound genistein. Health beneficial effects have been attributed to the consumption of this compound, but the fact that it has estrogen-like activity has raised doubts regarding its potential risk in infants, newborns, or in the fetus and placenta during pregnancy. This work is aimed at studying genistein effects on Ca2+ handling by smooth muscle cells of the human umbilical artery (HUA). Using fluorometric techniques, we found that in these cells genistein reduces the intracellular Ca2+ peak produced by serotonin. The same result could be demonstrated in absence of extracellular Ca2+, suggesting that the isoflavone reduces Ca2+ release from the sarcoplasmic reticulum. Force measurement experiments strengthen these results, since genistein reduced the peak force attained by intact HUA rings stimulated by serotonin in a Ca2+-free solution. Moreover, genistein induced the relaxation of HUA rings precontracted either with serotonin or a depolarizing high-extracellular K+ solution, hinting at a reduction of extracellular Ca2+ entry to the cell. This was confirmed by whole-cell patch-clamp experiments where it was shown that the isoflavone inhibits ionic currents through voltage-operated Ca2+ channels. In summary, we show that genistein inhibits two mechanisms that could increase intracellular Ca2+ in human umbilical smooth muscle cells, behaving in this way as a potential vasorelaxing substance of fetal vessels. Taking into account that genistein is able to cross the placental barrier, these data show that isoflavones may have important implications in the regulation of feto-maternal blood flow in pregnant women who consume soy-derived products as part of their meals. Facultad de Ciencias Exactas |
| Databáze: | OpenAIRE |
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