Contractile activity-specific transcriptome response to acute endurance exercise and training in human skeletal muscle
| Autor: | Daniil V. Popov, G. R. Gazizova, Olga Vinogradova, Oleg Gusev, Pavel A. Makhnovskii, E. A. Lysenko, Elena Shagimardanova |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism Quadriceps Muscle Mitochondrial Proteins Transcriptome Young Adult 03 medical and health sciences 0302 clinical medicine Endurance training Physiology (medical) Internal medicine medicine Humans Aerobic exercise RNA Messenger Muscle Skeletal Exercise Transcription factor business.industry Gene Expression Profiling Skeletal muscle Endurance Training 030104 developmental biology medicine.anatomical_structure Endocrinology business 030217 neurology & neurosurgery Muscle Contraction Transcription Factors |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 316:E605-E614 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.00449.2018 |
| Popis: | Reduction in daily activity leads to dramatic metabolic disorders, while regular aerobic exercise training is effective for preventing this problem. The purpose of this study was to identify genes that are directly related to contractile activity in human skeletal muscle, regardless of the level of fitness. Transcriptome changes after the one-legged knee extension exercise in exercised and contralateral nonexercised vastus lateralis muscle of seven men were evaluated by RNA-seq. Transcriptome change at baseline after 2 mo of aerobic training (5/wk, 1 h/day) was evaluated as well. Postexercise changes in the transcriptome of exercised muscle were associated with different factors, including circadian oscillations. To reveal transcriptome response specific for endurance-like contractile activity, differentially expressed genes between exercised and nonexercised muscle were evaluated at 1 and 4 h after the one-legged exercise. The contractile activity-specific transcriptome responses were associated only with an increase in gene expression and were regulated mainly by CREB/ATF/AP1-, MYC/MAX-, and E2F-related transcription factors. Endurance training-induced changes (an increase or decrease) in the transcriptome at baseline were more pronounced than transcriptome responses specific for acute contractile activity. Changes after training were associated with widely different biological processes than those after acute exercise and were regulated by different transcription factors (IRF- and STAT-related factors). In conclusion, adaptation to regular exercise is associated not only with a transient (over several hours) increase in expression of many contractile activity-specific genes, but also with a pronounced change (an increase or decrease) in expression of a large number of genes under baseline conditions. |
| Databáze: | OpenAIRE |
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