2-DE analysis of breast cancer cell lines 1833 and 4175 with distinct metastatic organ-specific potentials: Comparison with parental cell line MDA-MB-231
| Autor: | Jana Mládková, Aruna Vashishta, Irena Selicharová, Martin Fusek, Vaclav Vetvicka, Jiri Jiracek, Sujata Saraswat Ohri, Miloslav Sanda |
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| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Cell Heme binding protein 1 Cathepsin D Breast Neoplasms Biology Cathepsin B Mass Spectrometry Cell Line Tumor medicine Image Processing Computer-Assisted Humans Electrophoresis Gel Two-Dimensional Neoplasm Invasiveness Collagenases Neoplasm Metastasis Phosphoglycerate kinase 1 education education.field_of_study Gene Expression Profiling General Medicine Cell cycle Hydrogen-Ion Concentration Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology Cell culture Cancer research Female Peptides Annexin A2 |
| Zdroj: | Scopus-Elsevier |
| Popis: | Human MDA-MB-231 derived breast cancer cell lines 1833 and 4175 have different metastatic potentials in terms of their tissue tropisms and aggressiveness. Cell line 1833 is specifically metastatic to the bone. The highly aggressive cell line 4175 is specific to the lung. We performed 2-DE analysis of the cell lines. We found 16 significantly changed protein spots, 14 protein spots were identified. Expression of cathepsin D, triosephosphate isomerase, phosphoglycerate kinase 1, heme binding protein 1 and annexin 2 could be correlated with the in vitro aggressiveness of the respective cell lines. Interstitial collagenase and dimethylargininase 2 were exclusive to the cell line 1833 and might contribute to its bone specificity. Serpin B9, cathepsin B chain b, galectin 3 and HSP 27 were changed in the lung specific cell line 4175. The possible contribution of identified proteins to differences in metastatic behavior of the cell lines is discussed. |
| Databáze: | OpenAIRE |
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