Isolation and characterization of a generalized transducing phage for Xanthomonas campestris pv. campestris
| Autor: | M Kessel, Spencer A. Benson, M A Capage, B D Weiss |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Transposable element
Genome Viral Virus Replication Xanthomonas campestris Microbiology Xanthomonas campestris pv. campestris Bacteriophage Transduction (genetics) chemistry.chemical_compound Transduction Genetic Bacteriophages Molecular Biology Soil Microbiology Genetics biology Genetic transfer DNA Viruses Galactosides biology.organism_classification Molecular biology Mutagenesis Insertional chemistry Lytic cycle DNA Viral DNA Research Article |
| Zdroj: | Journal of bacteriology. 176(11) |
| ISSN: | 0021-9193 |
| Popis: | We have isolated and characterized a lytic double-stranded DNA Xanthomonas campestris pv. campestris bacteriophage (XTP1) capable of mediating generalized transduction. The phage transduces chromosomal markers at frequencies of 10(-5) to 10(-6) transductants per PFU. We demonstrated its genetic utility by the isolation and cotransduction of linked transposon insertions to a nonselectable locus, xgl, required for the cleavage of 5-bromo-3-chloro-indoyl-beta-D-galactoside and showed that rif and str alleles in X. campestris are 75% linked. One-step growth experiments showed that the latent and rise periods were each 2 h and the average burst size was 35. The DNA genome is approximately 180 kb, presumably modified in a sequence-specific manner, and may be covalently attached to protein(s). Electron micrographs show the phage particle to have an icosahedral head and contractile tail with tail fibers uniquely attached to a location 40 nm proximal from the end of the tail. |
| Databáze: | OpenAIRE |
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