An alternative, easy and reproducible method of stabilization and ligature-induced periodontitis in mouse
| Autor: | Rebeca De Souza Azevedo, Lucas N. de Queiroz, Geisoellen F. Araujo, Suiane S.C. Pereira, Bruno K. Robbs, Vinicius D'Ávila Bitencourt Pascoal, Priscilla Rodrigues Câmara |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Molar
Bone loss medicine.medical_treatment Clinical Biochemistry Method Dentistry 010501 environmental sciences 01 natural sciences Mandibular first molar Mice Immobilization 03 medical and health sciences Periodontal disease medicine Tooth loss Animal model Ligature lcsh:Science Dental alveolus ComputingMethodologies_COMPUTERGRAPHICS 030304 developmental biology 0105 earth and related environmental sciences Periodontitis 0303 health sciences business.industry Ligature-induced periodontitis Medicine and Dentistry medicine.disease Medical Laboratory Technology lcsh:Q medicine.symptom Ligation business |
| Zdroj: | MethodsX, Vol 6, Iss, Pp 2156-2165 (2019) MethodsX |
| ISSN: | 2215-0161 |
| Popis: | Graphical abstract Periodontal disease is one of the most common causes of tooth loss in the world. Ligature-induced is the most used method to study periodontitis. Here, we describe a alternative, easy and accessible experimental technique of ligation in mice. Twenty C57BL/6 female mice were divided in two groups, control and ligation. Ligature group (n = 10) was immobilized in a well described stabilization board and ligature was performed at the first molar using a new procedure here described in detail. Eight weeks later animals were euthanized, and periodontitis hallmarks were evaluated. Ligatures remained attached to the teeth in all animal during the hole experiment. The procedure induced a temporary loss of weight but no causalities or tooth loss. The animals affected by ligation in their molar teeth presented all periodontitis hallmarks, including alveolar bone loss, gingival retraction and inflammatory infiltrate in the studied region both macro and microscopically. The alternative method is low cost, easily reproducible, and induces all periodontitis hallmarks that are sustained until 8 weeks after placement. • Ligature-induced periodontitis in mouse is a powerful tool of research. • Methods describing the procedure in literature are difficult to reproduce. • A alternative stabilization and ligation procedure in mice is completely described here. |
| Databáze: | OpenAIRE |
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