Morphological dissection and cellular and transcriptome characterizations of bamboo pith cavity formation reveal a pivotal role of genes related to programmed cell death
| Autor: | Dingfan Cui, Jianyuan Feng, Yujun Wang, Xingyu Feng, Zhongru Li, Lin Guo, Yulong Ding, Chunxia Zhang, Zhangjun Fei, Xuepeng Sun, Qiang Wei, Chen Jiao |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Programmed cell death Bamboo Apoptosis Plant Science Genes Plant Poaceae 01 natural sciences Transcriptome 03 medical and health sciences Microscopy Electron Transmission Organelle fast growth asymmetrical growth In Situ Nick-End Labeling Pseudosasa japonica DNA Cleavage Cell wall modification programmed cell death Research Articles Plant stem reactive oxygen species calcium biology pith cavity Gene Expression Profiling biology.organism_classification Cell biology 030104 developmental biology RNA Plant Pith Agronomy and Crop Science hormones hormone substitutes and hormone antagonists 010606 plant biology & botany Biotechnology circulatory and respiratory physiology Research Article |
| Zdroj: | Plant Biotechnology Journal |
| ISSN: | 1467-7652 1467-7644 |
| Popis: | Summary Pith cavity formation is critical for bamboo to overcome the bending force during its fast growth; however, the underlying molecular mechanisms remain largely unknown. Multiple approaches, including anatomical dissection, mathematical modelling and transcriptome profiling, were employed in this study to investigate the biology of pith cavity formation in bamboo Pseudosasa japonica. We found that the corruption of pith tissue occurred sequentially and asymmetrically from the top‐centre of the internode down to the bottom, which might be caused by the combined effects of asymmetrical radial and axial tensile forces during shoot‐wall cell elongation and spiral growth of bamboo internodes. Programmed cell death (PCD) in pitch manifested by TUNEL positive nuclei, DNA cleavage and degraded organelles, and potentially regulated by ethylene and calcium signalling pathway, ROS burst, cell wall modification, proteolysis and nutrient recycle genes, might be responsible for pith tissue corruption of Ps. japonica. Although similar physiological changes and transcriptome profiles were found in different bamboo species, different formation rates of pith cavity were observed, which might be caused by different pith cells across the internode that were negatively correlated with the culm diameter. These findings provided a systematical view on the formation of bamboo pith cavity and revealed that PCD plays an important role in the bamboo pith cavity formation. |
| Databáze: | OpenAIRE |
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