Expression of coxsackievirus and adenovirus receptor in neointima of the rat carotid artery
| Autor: | Takuya Ozawa, Yoshifusa Aizawa, Yuko Hotta, Kaori Yoshida, Haruo Hanawa, Akimitsu Nasuno, Takashi Saigawa, Ryozo Kuwano, Yasser Osman, Kenichi Watanabe, Kiminori Kato, Ken Toba |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Neointima Intimal hyperplasia Vascular smooth muscle Biology Coxsackievirus Muscle Smooth Vascular Pathology and Forensic Medicine Rats Sprague-Dawley Proliferating Cell Nuclear Antigen medicine Animals RNA Messenger Receptor Cells Cultured Enterovirus Neointimal hyperplasia Hyperplasia Microscopy Confocal Reverse Transcriptase Polymerase Chain Reaction Cell adhesion molecule Endothelial Cells General Medicine musculoskeletal system medicine.disease biology.organism_classification Immunohistochemistry Molecular biology Rats Proliferating cell nuclear antigen Carotid Arteries Immunology cardiovascular system biology.protein Receptors Virus Carotid Artery Injuries Tunica Intima Tunica Media Cardiology and Cardiovascular Medicine Cell Adhesion Molecules human activities |
| Zdroj: | Cardiovascular Pathology. 13:79-84 |
| ISSN: | 1054-8807 |
| DOI: | 10.1016/s1054-8807(03)00137-6 |
| Popis: | Our previous study revealed that the coxsackievirus and adenovirus receptor (CAR) is a homophilic cell adhesion molecule and may function as a sensor of cell-cell interactions in the brain and damaged heart. In this study, we investigated if CAR expression is involved in the formation of neointimal hyperplasia using a balloon injury model of rat carotid artery. Cultured vascular smooth muscle cells (SMCs) from rat aorta were also studied. CAR antigen was constitutively detected in the endothelial cells (ECs) but not in SMCs before injury. On Day 5 after balloon injury, CAR was expressed strongly in the first layer of medial SMCs. Neointimal hyperplasia was observed on Day 7, and strong expressions of CAR concomitantly with proliferating cell nuclear antigen (PCNA) were obvious in the neointimal SMCs, while CAR in medial SMCs disappeared. The expression of CAR mRNA reached a peak on Day 7 and declined gradually to the basal levels. When the ECs regenerated on Day 14, CAR antigen was observed in the ECs but disappeared in the neointima. CAR together with PCNA was expressed abundantly in the proliferating SMCs in vitro and diminished in cells grown to a confluent state. The abundant expression of CAR in the neointima may facilitate an adenoviral gene therapy. |
| Databáze: | OpenAIRE |
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