Determination of Benzopyrene-Induced Lung Inflammatory and Cytotoxic Injury in a Chemical Gradient-Integrated Microfluidic Bronchial Epithelium System
| Autor: | Chang Tian, Wenming Liu, Yuanqing Wei, Jinyi Wang, Huaisheng Wang, Fen Zhang, Songqin Liu, Kan Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Cell Bronchi Enzyme-Linked Immunosorbent Assay Bioengineering Environmental pollution 01 natural sciences Epithelium Cell Line 03 medical and health sciences chemistry.chemical_compound Tissue engineering Lab-On-A-Chip Devices medicine Humans Secretion Benzopyrenes Instrumentation Fluid Flow and Transfer Processes chemistry.chemical_classification Reactive oxygen species Microscopy Confocal Lung Caspase 3 Interleukin-6 Tumor Necrosis Factor-alpha Process Chemistry and Technology Interleukin-8 010401 analytical chemistry Epithelial Cells Equipment Design 0104 chemical sciences Cell biology 030104 developmental biology medicine.anatomical_structure Cytokine Microscopy Fluorescence chemistry Benzopyrene Reactive Oxygen Species |
| Zdroj: | ACS Sensors. 3:2716-2725 |
| ISSN: | 2379-3694 |
| DOI: | 10.1021/acssensors.8b01370 |
| Popis: | Environmental pollution is one of the largest sources responsible for human diseases and premature death worldwide. However, the methodological development of a spatiotemporally controllable and high-throughput investigation of the environmental pollution-induced biological injury events is still being explored. In this study, we describe a chemical gradient generator-aided microfluidic cell system for the dynamic study of representative environmental pollutant-induced bronchial epithelium injury in a throughput manner. We demonstrated the stability and reliability of operation-optimized microfluidic system for precise and long-term chemical gradient production. We also performed a microenvironment-controlled microfluidic bronchial epithelium construction with high viability and structure integration. Moreover, on-chip investigation of bronchial epithelium injury by benzopyrene stimulation with various concentrations can be carried out in the single device. The varying bronchial inflammatory and cytotoxic responses were temporally monitored and measured based on the well-established system. The benzopyrene directionally led the bronchial epithelium to present observable cell shrinkage, cytoskeleton disintegration, Caspase-3 activation, overproduction of reactive oxygen species, and various inflammatory cytokine (TNF-α, IL-6, and IL-8) secretion, suggesting its significant inflammatory and cytotoxic effects on respiratory system. We believe the microfluidic advancement has potential applications in the fields of environmental monitoring, tissue engineering, and pharmaceutical development. |
| Databáze: | OpenAIRE |
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