| Autor: |
Joseph R. Testa, Siddharth Balachandran, Raja M. Flores, Suresh C. Jhanwar, Suresh Ramanathan, Eric A. Ross, Lauren A. Fox, Vladimir Khazak, Ting Zhang, Anna-Mariya Kukuyan, Craig W. Menges, Suraj Peri, Mitchell Cheung, Eleonora Sementino, Yinfei Tan |
| Rok vydání: |
2023 |
| DOI: |
10.1158/1078-0432.22473981.v1 |
| Popis: |
Expression of RIPK3 induced activation of MLKL and cell death, which was rescued by treatment with GSK'872. Transduction of M12 cells (C) and M22 cells (D) with lentivirus expressing shMLKL or shGFP and then selected for 72 h with puromycin. Stable cells expressing shMLKL or shGFP were then infected with WPI or WPI expressing RIPK3. Immunoblotting and cell viability were performed after 48 h to detect the activation of MLKL. Expression of RIPK3 induced necroptosis, which was rescued by MLKL knockdown. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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