Multiparametric Profiling of Single Nanoscale Extracellular Vesicles by Combined Atomic Force and Fluorescence Microscopy : Correlation and Heterogeneity in Their Molecular and Biophysical Features
| Autor: | Fredrik Stridfeldt, Siddharth S. Sahu, Apurba Dev, Carolina Paba, Doste R Mamand, Sara Cavallaro, Federico Pevere, Samir El Andaloussi, Jan Linnros, André Görgens, Dhanu Gupta |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Stromal cell
Tetraspanins size profiling Cell- och molekylärbiologi Cell Population Biophysics Medizin Cell Communication 02 engineering and technology mechanical properties protein profiling 010402 general chemistry fluorescence microscopy 01 natural sciences Biomaterials Extracellular Vesicles Tetraspanin medicine Fluorescence microscope Humans General Materials Science Child education education.field_of_study General Chemistry single vesicle profiling 021001 nanoscience & nanotechnology Biofysik 0104 chemical sciences medicine.anatomical_structure Microscopy Fluorescence Membrane protein AFM extracellular vesicles 0210 nano-technology Cell and Molecular Biology Intracellular Biotechnology CD81 |
| Popis: | Being a key player in intercellular communications, nanoscale extracellular vesicles (EVs) offer unique opportunities for both diagnostics and therapeutics. However, their cellular origin and functional identity remain elusive due to the high heterogeneity in their molecular and physical features. Here, for the first time, multiple EV parameters involving membrane protein composition, size and mechanical properties on single small EVs (sEVs) are simultaneously studied by combined fluorescence and atomic force microscopy. Furthermore, their correlation and heterogeneity in different cellular sources are investigated. The study, performed on sEVs derived from Human Embryonic Kidney 293, Cord Blood Mesenchymal Stromal and Human Acute Monocytic Leukemia cell lines, identifies both common and cell line-specific sEV subpopulations bearing distinct distributions of the common tetraspanins (CD9, CD63 and CD81) and biophysical properties. Although the tetraspanin abundances of individual sEVs are independent of their sizes, the expression levels of CD9 and CD63 are strongly correlated. A sEV population co-expressing all the three tetraspanins in relatively high abundance, however, having on average diameters |
| Databáze: | OpenAIRE |
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