Quorum sensing mediated response of Achromobacter denitrificans SP1 towards prodigiosin production under phthalate stress
| Autor: | Pradeep Selvanesan, Harilal Cherukara Chellappan, Julia Garvasis, Hareesh Erandapurathukadumana Sreedharan |
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| Rok vydání: | 2019 |
| Předmět: |
endocrine system
Homoserine Acyl-Butyrolactones Applied Microbiology and Biotechnology Prodigiosin 03 medical and health sciences chemistry.chemical_compound Plasticizers Stress Physiological Diethylhexyl Phthalate Incubation 030304 developmental biology 0303 health sciences biology 030306 microbiology Vanillin Phthalate food and beverages Achromobacter denitrificans Quorum Sensing General Medicine biology.organism_classification Quorum sensing Biodegradation Environmental chemistry Biochemistry Benzaldehydes Bacteria |
| Zdroj: | Journal of basic microbiologyREFERENCES. 60(9) |
| ISSN: | 1521-4028 |
| Popis: | Quorum sensing is a density-dependent chemical process between bacteria, which may be intergenus or intragenus. N-acyl homoserine lactones (HSLs) are a type of small signaling molecules associated with Gram-negative bacteria for monitoring their own population density. The present study unveils the mechanism of HSLs in Achromobacter denitrificans SP1 while transforming di(2-ethylhexyl) phthalate (DEHP) into prodigiosin in a simple basal salt medium. The primary detection of HSLs was done by the colorimetric method. Fourier-transform infrared spectroscopy and liquid chromatography-mass spectrometry-quadrupole time-of-flight confirmed and identified the HSLs. The maximum production of HSLs was observed between 24 and 72 h of incubation, which is noted to be a peak time of DEHP degradation. A total of 57.2% of DEHP was degraded within 30 h and complete degradation was observed within 72 h of incubation. Regulation in the synthesis of various acyl-HSL molecules, viz. 3OC6-HSL in the initial stage of DEHP stress, 3OC8-HSL, and C10-HSL during the time of degradation and 3OC12-HSL on completion of degradation was noticed. The role of HSLs on the production of prodigiosin was confirmed using vanillin as an HSL inhibitor. Through the selective activation of HSL molecules, A. denitrificans SP1 sustain the changing stressful conditions. Supplementation of acyl-HSL signal molecules may boost up the efficacy of A. denitrificans SP1 in both DEHP degradation and prodigiosin production which offers great potential towards the management of DEHP containing plastic wastes. |
| Databáze: | OpenAIRE |
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