Enhanced proliferative capacity of human preadipocytes achieved by an optimized cultivating method that induces transient activity of hTERT
| Autor: | Kristina Mäemets-Allas, Claudia G. Cárdenas-León, Viljar Jaks, Kati Kuuse, Luis A. Salazar-Olivo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adipogenesis Cell Survival Chemistry Proliferative capacity Mesenchymal stem cell Cell Culture Techniques Biophysics Cell Biology Biochemistry Cell Line Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine SOX2 030220 oncology & carcinogenesis Adipocytes Humans Telomerase reverse transcriptase Telomerase Molecular Biology Cell Proliferation |
| Zdroj: | Biochemical and Biophysical Research Communications. 529:455-461 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2020.06.019 |
| Popis: | Human mesenchymal stromal cells (MSC) are an important tool for basic and translational research. Large amounts of MSC are required for in vitro and in vivo studies, however, the limited life-span and differentiation ability in vitro hamper their optimal use. Here we report that 1:1 mixture of L15 and mTeSR1 culture media increased the life-span of IPI-SA3-C4, a normal non-immortalized human subcutaneous preadipocyte strain by 20% while retaining their adipogenic capacity and stable karyotype. The increased proliferative capacity was accompanied by increased expression of the stem markers POU5F1, SOX2, MYC and hTERT, and inhibition of hTERT activity abolished the growth advantage of L15-mTeSR1. Consequently, the described MSC culture would considerably enhance the utility of MSC for in vitro studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect