Molecular diversification of the seminal fluid proteome in a recently diverged passerine species pair
Autor: | Fabrice Eroukhmanoff, Mark Ravinet, Emma Whittington, Steve Dorus, Kirill Borziak, Melissah Rowe, Glenn-Peter Sætre |
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Přispěvatelé: | Animal Ecology (AnE) |
Rok vydání: | 2019 |
Předmět: |
Male
Proteomics Genetic Speciation cryptic female choice Genome sperm competition reproduction selective sweep Avian Proteins Evolution Molecular positive selection Molecular evolution biology.animal Genetics Animals Gene Regulatory Networks Molecular Biology Sperm competition Discoveries Phylogeny Ecology Evolution Behavior and Systematics fertility Whole Genome Sequencing biology Immunity Plan_S-Compliant_NO Reproductive isolation Spermatozoa Passerine Female sperm storage Evolutionary biology international Proteome Selective sweep Sparrows |
Zdroj: | Molecular Biology and Evolution, 37(2):msz235, 488-506. Oxford University Press Molecular Biology and Evolution |
ISSN: | 0737-4038 |
Popis: | Seminal fluid proteins (SFPs) mediate an array of postmating reproductive processes that influence fertilization and fertility. As such, it is widely held that SFPs may contribute to postmating, prezygotic reproductive barriers between closely related taxa. We investigated seminal fluid (SF) diversification in a recently diverged passerine species pair (Passer domesticus and Passer hispaniolensis) using a combination of proteomic and comparative evolutionary genomic approaches. First, we characterized and compared the SF proteome of the two species, revealing consistencies with known aspects of SFP biology and function in other taxa, including the presence and diversification of proteins involved in immunity and sperm maturation. Second, using whole-genome resequencing data, we assessed patterns of genomic differentiation between house and Spanish sparrows. These analyses detected divergent selection on immunity-related SF genes and positive selective sweeps in regions containing a number of SF genes that also exhibited protein abundance diversification between species. Finally, we analyzed the molecular evolution of SFPs across 11 passerine species and found a significantly higher rate of positive selection in SFPs compared with the rest of the genome, as well as significant enrichments for functional pathways related to immunity in the set of positively selected SF genes. Our results suggest that selection on immunity pathways is an important determinant of passerine SF composition and evolution. Assessing the role of immunity genes in speciation in other recently diverged taxa should be prioritized given the potential role for immunity-related proteins in reproductive incompatibilities in Passer sparrows. |
Databáze: | OpenAIRE |
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