Butyl benzyl phthalate: effects on immune responses to ovalbumin in mice
| Autor: | Rebecca J. Dearman, Lorna Beresford, Laura Bailey, Catherine J. Betts, Helen Theresa Caddick, Ian Kimber |
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| Rok vydání: | 2008 |
| Předmět: |
Allergy
Ovalbumin medicine.medical_treatment Administration Topical Phthalic Acids Enzyme-Linked Immunosorbent Assay Toxicology Immunoglobulin E medicine.disease_cause chemistry.chemical_compound Mice Immune system Allergen Adjuvants Immunologic Plasticizers medicine Animals Mice Inbred BALB C biology Dose-Response Relationship Drug Passive Cutaneous Anaphylaxis Phthalate Organ Size Allergens medicine.disease chemistry Liver Immunoglobulin G Immunology biology.protein Environmental Pollutants Female Antibody Adjuvant Injections Intraperitoneal |
| Zdroj: | Journal of applied toxicology : JAT. 29(2) |
| ISSN: | 1099-1263 |
| Popis: | During recent decades the prevalence of IgE-mediated (atopic) allergic diseases in Western Europe and the USA has been increasing dramatically. It has been suggested that one possible cause is the presence in the environment of chemicals that may act as adjuvants, enhancing immune and allergic responses. Certain commonly used phthalate plasticizers such as butyl benzyl phthalate (BBP) have been implicated in this way. In the current experiments, the impact of BBP, applied by a physiologically relevant exposure route, on the vigour of immune responses induced in BALB/c strain mice has been examined. Mice were immunized via subcutaneous injection with the reference allergen ovalbumin (OVA) and received concurrent topical treatment with doses of BBP that induced significant changes in liver weight. The generation of specific anti-OVA IgE and IgG1 antibodies was measured by passive cutaneous anaphylaxis and by enzyme-linked immunosorbant assays, respectively. Topical administration of BBP was without impact on anti-OVA IgE antibody responses, regardless of whether BBP was applied locally or distant to the site of OVA immunization. However, same-site treatment with high-dose BBP (100 mg) did result in a modest elevation in anti-OVA IgG1 antibody production, a subclass of antibody used as a surrogate marker of IgE responses. Taken together with human exposure data, these results suggest that the doses of phthalate encountered in the home environment are unlikely to be a major factor contributing to the increased incidence of asthma and allergy in the developed world. |
| Databáze: | OpenAIRE |
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