Increased BnaMFT-transcript level is associated with secondary dormancy in oilseed rape (Brassica napus L.)
| Autor: | Ying Zhou, Xin Yi, XiangXiang Zhao, Zi-wei Tang, Lei Liu, Tang Tang, FuXia Liu, Wen-qi Fan, Chen Guimin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Agriculture (General) Brassica Plant Science 01 natural sciences Biochemistry S1-972 chemistry.chemical_compound Food Animals Transcription (biology) Botany MFT primary dormancy Abscisic acid Gene Ecology biology secondary dormancy fungi Brassica napus Seed dormancy food and beverages Promoter 04 agricultural and veterinary sciences biology.organism_classification chemistry Germination 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Dormancy DELAY OF GERMINATION 1 (DOG1) Animal Science and Zoology Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 19, Iss 6, Pp 1565-1576 (2020) |
| ISSN: | 2095-3119 |
| Popis: | Brassica napus cultivars have little or no primary dormancy; however, they are prone to secondary dormancy induction. Secondary dormant seeds can produce volunteer plants, which can result in genetic contamination, reduced quality and biosafety issues. However, information regarding the molecular mechanism underlying secondary dormancy is limited. The MOTHER OF FT AND TFL1 (MFT) gene, which is evolutionarily conserved in the plant kingdom, acts in a complex gene network in the seed dormancy or germination processes. In this study, we identified four B. napus genes that share high homology with AtMFT, named as BnaMFT. Analyses of cis-acting elements showed that BnaMFT promoters contain multiple seed-specific regulatory elements, and various stress- and hormone-responsive elements. Further experiments validated that BnaMFTs were specifically expressed during seed maturation and in the dry seed, with peaks at 35–42 days after pollination. BnaMFTs were not sufficient for primary dormancy; however, they were significantly enhanced by secondary dormancy induction with PEG6000 treatment. Moreover, BnaMFT transcripts were elevated by treatment with abscisic acid (ABA), which is known to be accumulated during secondary dormancy. These results collectively suggest that increased BnaMFT transcription levels are associated with secondary dormancy induction in an ABA-dependent manner in B. napus. |
| Databáze: | OpenAIRE |
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