Tissue-specific transcriptional regulation of epithelial/endothelial and mesenchymal markers during renovascular hypertension

Autor:	Gabriela de Souza Rodrigues de Queiroz, Bianca Caruso Moreira, Regiane de Freitas Formigari, Camila Andréa de Oliveira, Bruna Fontana Thomazini, Maíra Felonato, Marcelo Augusto Marreto Esquisatto, Armindo Antonio Alves, Leandro Ricardo Ferraz
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Male Transcriptional Activation Cancer Research medicine.medical_specialty Hypertension Renal two-kidney one-clip model medicine.disease_cause Biochemistry Renovascular hypertension Proinflammatory cytokine Internal medicine Genetics medicine Animals oxidative stress Rats Wistar Molecular Biology business.industry Mesenchymal stem cell Interleukin Articles medicine.disease Antigens Differentiation Rats Endocrinology Blood pressure Oncology Organ Specificity epithelial/endothelial-to-mesenchymal transition gene expression Molecular Medicine Hepatocyte growth factor Tumor necrosis factor alpha business Oxidative stress cadherins medicine.drug
Zdroj:	Molecular Medicine Reports
ISSN:	1791-3004 1791-2997
Popis:	Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition are processes that can occur under different biological conditions, including tissue healing due to hypertension and oxidative stress. The purpose of the present study was to evaluate the differences in gene expression of epithelial/endothelial and mesenchymal markers in different tissues. A two-kidney, one-clip (2K1C) renovascular hypertension rat model was used. Hypertension was induced by the clipping of the left renal artery; the rats were randomized into sham and 2K1C groups and monitored for up to 4 weeks. The gene expressions of E-cadherin (E-cad), N-cadherin (N-cad), α-smooth muscle actin (α-SMA), collagen I (COL1A1), collagen III (COL3A1) and hepatocyte growth factor (HGF) were determined by reverse transcription-PCR. The levels of the cytokines transforming growth factor-β1, tumor necrosis factor-α, interleukin (IL)-4, IL-6 and IL-10 were evaluated using ELISAs. The levels of thiobarbituric acid reactive substances and thiol groups were measured to evaluate oxidative stress. All analyses were performed on the liver, heart and kidneys tissues of sham and model rats. The 2K1C animals exhibited a higher systolic blood pressure, as well as cardiac hypertrophy and atrophy of the left kidney. Fibrotic alterations in the heart and kidneys were observed, as was an increase in the collagen fiber areas, and higher levels of inflammatory cytokines, which are associated with the increased expression of fibroproliferative and anti-fibrotic genes. Renovascular hypertension regulated epithelial/endothelial and mesenchymal markers, including E-cad, N-cad, α-SMA and COL1A1 in the kidneys and heart. EMT in the kidneys was mediated by an increased level of inflammatory and profibrotic cytokines, as well as by oxidative stress. The data in the present study suggested that the expression of epithelial/endothelial and mesenchymal markers are differentially regulated by hypertension in the liver, heart and kidneys.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53fc6bc1e8b82c4dda37e469ab55b2df http://europepmc.org/articles/PMC6797995 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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