PEBP2—A Modulator of Polyoma DNA Replication
| Autor: | Ze'ev Ronai, Susan E. Rutberg, Lisa Dolan |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
DNA Replication
Ultraviolet Rays DNA damage Molecular Sequence Data Core Binding Factor Alpha 1 Subunit Biology Virus Replication DNA-binding protein Core Binding Factor beta Subunit Mice chemistry.chemical_compound Control of chromosome duplication Genetics Animals Nuclear protein Molecular Biology Cells Cultured Base Sequence DNA replication Cell Biology General Medicine Transfection Molecular biology Rats DNA-Binding Proteins Transcription Factor AP-2 Viral replication chemistry DNA Viral Polyomavirus DNA Transcription Factors |
| Zdroj: | DNA and Cell Biology. 13:865-874 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.1994.13.865 |
| Popis: | Previously, we have shown that integrated copies of polyoma DNA can be induced to replicate in rat fibroblasts (H3 cells) exposed to a DNA-damaging agent. In the current study, we demonstrate that UV-irradiation of mouse fibroblasts (WOP cells), transiently transfected with polyoma DNA, results in repression of polyoma replication. Cotransfection of oligomers representing wild-type but not mutated forms of the PEBP2 target sequence restored levels of viral replication indicating a role of PEBP2 binding proteins in mediating this effect. DNA-binding assays revealed that a different subset of complexes was formed with the PEBP2 target sequence when nuclear proteins from sham and UV-irradiated WOP and H3 cells were compared, suggesting that the activities of PEBP2 binding proteins are differentially regulated upon UV-irradiation in these two cell types. The ability of PEBP2 to modulate polyoma replication following UV-irradiation in WOP cells suggests a potential role of PEBP2 proteins in the cellular response to DNA damage. |
| Databáze: | OpenAIRE |
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