Ratiometric Barcoding for Mass Cytometry
| Autor: | Xu Wu, William W. Kwok, I-Che Wu, Daniel T. Chiu, Li Wu, Quinn DeGottardi, Jiangbo Yu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cellular targeting Cell Separation 01 natural sciences Article Analytical Chemistry 03 medical and health sciences High-Throughput Screening Assays Cell separation Humans Mass cytometry Radiometry Fluorescent Dyes 010405 organic chemistry Extramural Chemistry Flow Cytometry Endocytosis 0104 chemical sciences Molecular analysis 030104 developmental biology Biochemistry Semiconductors Leukocytes Mononuclear MCF-7 Cells Leukocyte Common Antigens |
| Zdroj: | Analytical chemistry. 90(18) |
| ISSN: | 1520-6882 |
| Popis: | Barcoding is of importance for high-throughput cellular and molecular analysis. A ratiometric barcoding strategy using lanthanides-coordinated polymer dots (Ln-Pdots) was developed for mass cytometric analysis. By using three metal isotopes and four ratio intensity levels, 16 barcodes were generated to code, and later decode, cell samples in mass cytometry. The ratiometric Ln-Pdots barcodes not only provided high mass signal intensities, but also eliminated the bias caused by different concentrations of the labeling reagents/barcodes and run- to-run differences in cell labeling efficiency. The ability to distinguish clearly the 16 sets of labeled MCF-7 cells with mass cytometry demonstrated the excellent resolving power of the ratiometric Ln-Pdots barcodes. Furthermore, the results from barcoding PBMCs samples via CD45 specific cellular targeting indicated that the ratiometric Ln-Pdots barcodes could facilitate mass cytometry in high-throughput and multiplexed analysis, especially with precious human samples. |
| Databáze: | OpenAIRE |
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