Virion Proteins of Kaposi's Sarcoma-Associated Herpesvirus
| Autor: | Fanxiu Zhu, Lijun Wu, Jae Min Chong, Yan Yuan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
viruses
Immunology Molecular Sequence Data Biology medicine.disease_cause Microbiology Viral Proteins Viral Envelope Proteins Virology Cell Line Tumor Terminology as Topic medicine Gammaherpesvirinae Humans Amino Acid Sequence Kaposi's sarcoma-associated herpesvirus Peptide sequence Gel electrophoresis chemistry.chemical_classification Structure and Assembly Virion virus diseases Viral tegument biochemical phenomena metabolism and nutrition biology.organism_classification Capsid chemistry Virion assembly Insect Science Herpesvirus 8 Human Capsid Proteins Glycoprotein |
| Popis: | The proteins that compose a herpesvirus virion are thought to contain the functional information required for de novo infection, as well as virion assembly and egress. To investigate functional roles of Kaposi's sarcoma-associated herpesvirus (KSHV) virion proteins in viral productive replication and de novo infection, we attempted to identify virion proteins from purified KSHV by a proteomic approach. Extracellular KSHV virions were purified from phorbol-12-tetradecanoate-13-acetate-induced BCBL-1 cells through double-gradient ultracentrifugation, and their component proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirty prominent protein bands were excised and subjected to high-performance liquid chromatography ion trap mass spectrometric analysis. This study led to the identification of 24 virion-associated proteins. These include five capsid proteins, eight envelope glycoproteins, six tegument proteins, and five proteins whose locations in the virions have not yet been defined. Putative tegument proteins encoded by open reading frame 21 (ORF21), ORF33, and ORF45 were characterized and found to be resistant to protease digestion when purified virions were treated with trypsin, confirming that they are located within the virion particles. The ORF64-encoded large tegument protein was found to be associated with capsid but sensitive to protease treatment, suggesting its unique structure and array in KSHV virions. In addition, cellular β-actin and class II myosin heavy chain type A were found inside KSHV virions and associated with tegument-capsid structure. Identification of KSHV virion proteins makes it possible to study the functional roles of these virion proteins in KSHV replication and pathogenicity. |
| Databáze: | OpenAIRE |
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