Antitumor activity of emodin against pancreatic cancer depends on its dual role: promotion of apoptosis and suppression of angiogenesis
| Autor: | Hui Chen, Wei-Tian Wei, Hai-Bin Liu, Hong-Chun Guo, Zhong-Lin Ni, Dian-Lei Liu, Hongfei Tong, Shengzhang Lin, Zhao-Hong Wang, Kangjie Chen |
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| Rok vydání: | 2012 |
| Předmět: |
Phytochemistry
Anatomy and Physiology Angiogenesis Phytopharmacology Phytochemicals Cancer Treatment lcsh:Medicine Angiogenesis Inhibitors Apoptosis Pharmacology Neovascularization chemistry.chemical_compound Mice Enos Immune Physiology Molecular Cell Biology Drug Discovery Basic Cancer Research lcsh:Science Mice Inbred BALB C Multidisciplinary biology Neovascularization Pathologic Cell Death Chemistry Vascular Endothelial Growth Factors NF-kappa B Tumor Burden Gene Expression Regulation Neoplastic Matrix Metalloproteinase 9 Oncology Matrix Metalloproteinase 2 Cytokines Medicine Female Antiangiogenesis Therapy medicine.symptom medicine.drug Research Article Drugs and Devices Emodin Drug Research and Development Nitric Oxide Synthase Type III Immunology Mice Nude Antineoplastic Agents Gastroenterology and Hepatology Complementary and Alternative Medicine In vivo Pancreatic cancer Cell Line Tumor medicine Animals Humans Biology Pancreas lcsh:R Chemotherapy and Drug Treatment biology.organism_classification medicine.disease Xenograft Model Antitumor Assays Gemcitabine Enzyme Activation Pancreatic Neoplasms Immune System lcsh:Q Clinical Immunology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 8, p e42146 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Background Emodin has been showed to induce apoptosis of pancreatic cancer cells and inhibit tumor growth in our previous studies. This study was designed to investigate whether emodin could inhibit the angiogenesis of pancreatic cancer tissues and its mechanism. Methodology/Principal Finding In accordance with our previous study, emodin inhibited pancreatic cancer cell growth, induced apoptosis, and enhanced the anti-tumor effect of gemcitabine on pancreatic caner cells in vitro and in vivo by inhibiting the activity of NF-κB. Here, for the first time, we demonstrated that emodin inhibited tumor angiogenesis in vitro and in implanted pancreatic cancer tissues, decreased the expression of angiogenesis-associated factors (NF-κB and its regulated factors VEGF, MMP-2, MMP-9, and eNOS), and reduced eNOS phosphorylation, as evidenced by both immunohistochemistry and western blot analysis of implanted tumors. In addition, we found that emodin had no effect on VEGFR expression in vivo. Conclusions/Significance Our results suggested that emodin has potential anti-tumor effect on pancreatic cancer via its dual role in the promotion of apoptosis and suppression of angiogenesis, probably through regulating the expression of NF-κB and NF-κB-regulated angiogenesis-associated factors. |
| Databáze: | OpenAIRE |
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