Generation of a human control iPSC line with a European mitochondrial haplogroup U background
| Autor: | Francisco Zurita, Cristina González-Páramos, M. Esther Gallardo, Ana Moreno-Izquierdo, Teresa Galera, Rafael Garesse, Agustín F. Fernández, Mario F. Fraga |
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| Přispěvatelé: | UAM. Departamento de Bioquímica, Instituto de Investigaciones Biomédicas 'Alberto Sols' (IIBM), Universidad Autónoma de Madrid, Comunidad de Madrid, Ministerio de Educación, Cultura y Deporte (España), European Commission, Instituto de Salud Carlos III |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Medicina Cellular differentiation Cell Culture Techniques Mitochondrion Cell Line Kruppel-Like Factor 4 03 medical and health sciences stomatognathic system Humans Induced pluripotent stem cell Medicine(all) Genetics iPSC biology Haplotype Cell Differentiation Cell Biology General Medicine IPS cells biology.organism_classification DNA Fingerprinting Sendai virus Mitochondria Europe iPS cells Induced pluripotent stem cells 030104 developmental biology Haplotypes IPSC POLG KLF4 Karyotyping embryonic structures Defects of intergenomic communication Reprogramming Developmental Biology Human mitochondrial DNA haplogroup |
| Zdroj: | WOS:000375559500018 RUO. Repositorio Institucional de la Universidad de Oviedo instname Digital.CSIC. Repositorio Institucional del CSIC Biblos-e Archivo. Repositorio Institucional de la UAM |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2015.12.010 |
| Popis: | Human iPSC line N44SV.5 was generated from primary normal human dermal fibroblasts belonging to the European mitochondrial haplogroup U. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus. This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional Development Fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (grant number S2010/BMD-2402 to R.G); T.G. receives grant support from the Universidad Autónoma de Madrid, FPI-UAM and F.Z.D. from the Ministerio de Educación, Cultura y Deporte, grant number FPU13/00544. M.E.G. is staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER). |
| Databáze: | OpenAIRE |
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