Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: Homology to Epstein-Barr virus open reading frame BCRFI
| Autor: | Kevin W. Moore, David Fiorentino, J E deVries, M N Dang, Robert A. Kastelein, Maria Grazia Roncarolo, R de Waal-Malefyt, Pedro L. Vieira, Tim R. Mosmann, K E Johnson |
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| Předmět: |
endocrine system
Herpesvirus 4 Human Molecular Sequence Data Biology Molecular cloning Transfection Polymerase Chain Reaction Virus Cell Line Interferon-gamma Mice Open Reading Frames Complementary DNA Sequence Homology Nucleic Acid Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence Multidisciplinary Interleukins Nucleic acid sequence Granulocyte-Macrophage Colony-Stimulating Factor DNA Molecular biology Recombinant Proteins Interleukin-10 Interleukin 10 Open reading frame Cell culture Leukocytes Mononuclear Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading frame in the Epstein-Barr virus, BCRFI. hIL-10 and the BCRFI product inhibit cytokine synthesis by activated human peripheral blood mononuclear cells and by a mouse Th1 clone. Both hIL-10 and mouse IL-10 sustain the viability of a mouse mast cell line in culture, but BCRFI lacks comparable activity in this assay, suggesting that BCRFI may have conserved only a subset of hIL-10 activities. |
| Databáze: | OpenAIRE |
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