Transformation of Mollicutes with single-stranded Tn4001 DNA
| Autor: | Paul A. Kapke, F C Minion, Knudtson Kl |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Mycoplasma gallisepticum
Genetics Transposable element DNA Bacterial biology Models Genetic Inverted repeat Base pair Chromosome DNA Single-Stranded biology.organism_classification Molecular biology chemistry.chemical_compound Plasmid Mycoplasma chemistry Mollicutes DNA Transposable Elements Transformation Bacterial Molecular Biology DNA Acholeplasma |
| Zdroj: | Plasmid. 32(1) |
| ISSN: | 0147-619X |
| Popis: | Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log lower frequency than did A. oculi ISM1499. Restriction enzyme digestion of single-stranded DNA indicated homologous base pairing in the inverted repeat regions, which could account for the transpositional activity of single-stranded DNA. |
| Databáze: | OpenAIRE |
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