Localization of LGR7 Gene Expression in Adult Mouse Brain Using LGR7 Knock-out/LacZKnock-in Mice: Correlation with LGR7 mRNA Distribution
| Autor: | Tanya C. D. Burazin, Sherie Ma, Ross A. D. Bathgate, Loretta Piccenna, Sietse Mosselman, Andrew L. Gundlach, Pei-Juan Shen, Jan A. Gossen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cingulate cortex
Aging medicine.medical_specialty Hippocampus Mice Transgenic Biology Hippocampal formation General Biochemistry Genetics and Molecular Biology Receptors G-Protein-Coupled Mice History and Philosophy of Science Cortex (anatomy) Internal medicine medicine Animals RNA Messenger Transgenes In Situ Hybridization Relaxin Gene Expression Profiling General Neuroscience Subiculum Brain beta-Galactosidase Olfactory bulb Cell biology medicine.anatomical_structure Endocrinology Female Relaxin receptor |
| Zdroj: | Annals of the New York Academy of Sciences. 1041:197-204 |
| ISSN: | 0077-8923 |
| DOI: | 10.1196/annals.1282.030 |
| Popis: | Knowledge of the distribution of the relaxin receptor, LGR7, in the brain provides a basis for studies of the physiologic actions of relaxin. LGR7 knock-out (KO) mice were produced by the in-frame replacement of LGR7 exon 10 and 11 with a LacZ-reporter cassette (knock-in [KI]), and in this study we used LGR7-KO/LacZ-KI mice to determine the regional/cellular distribution of LGR7 gene expression in adult mouse brain by assessing beta-galactosidase activity in perfusion-fixed sections. High densities of beta-galactosidase-positive neurons were detected in anterior olfactory and claustrum/endopiriform nuclei, deep layers of cortex (particularly somatosensory), and the subiculum. Low to moderate densities were detected in olfactory bulb (periglomerular layer), cingulate cortex, subfornical organ, hippocampal CA2/dentate hilus, amygdala, hypothalamus, and thalamus. This LGR7/LacZ expression appears to recapitulate that of native LGR7 in wild-type mice and provides a model to further investigate the phenotype of LGR7-responsive neurons in the brain and to help reveal functions associated with central relaxin signaling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text