Genes required for mitotic spindle assembly in Drosophila S2 cells
| Autor: | Sarah S. Goodwin, Roy Wollman, Nan Zhang, Ronald D. Vale, Gohta Goshima, Jonathan M. Scholey, Nico Stuurman |
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| Rok vydání: | 2007 |
| Předmět: |
Aurora B kinase
Mitosis Genes Insect Spindle Apparatus Biology Microtubules Spindle pole body Chromosomes Article Cell Line Tubulin Image Processing Computer-Assisted Animals Drosophila Proteins Kinetochores Metaphase Centrosome Multidisciplinary Kinetochore Microtubule organizing center Cell biology Spindle apparatus Spindle checkpoint Drosophila melanogaster Phenotype RNA Interference |
| Zdroj: | Science (New York, N.Y.). 316(5823) |
| ISSN: | 1095-9203 |
| Popis: | The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled. |
| Databáze: | OpenAIRE |
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