Development of a new metastatic human breast carcinoma xenograft line
Autor: | Rajeshwari R. Mehta, J. M. Graves, T. K. Das Gupta, Anne Shilkaitis |
---|---|
Rok vydání: | 1998 |
Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Blotting Western Transplantation Heterologous Cell Mammary gland Mice Nude Breast Neoplasms Biology Metastasis Mice In vivo Tumor Cells Cultured Carcinoma medicine Animals Humans skin and connective tissue diseases Mice Inbred BALB C Matrigel Estradiol Carcinoma Ductal Breast medicine.disease Neoplasm Proteins Transplantation Drug Combinations medicine.anatomical_structure Oncology Female Proteoglycans Collagen Laminin Breast carcinoma Cell Division Neoplasm Transplantation Research Article |
Zdroj: | British Journal of Cancer |
ISSN: | 1532-1827 0007-0920 |
Popis: | Xenografts originated from human tumours offer the most appropriate research material for in vivo experimental research. However, primary human breast carcinomas are difficult to grow when transplanted in athymic mice: tumour take is less than 15%. Recently, we have achieved 60% tumour take by injecting tumour cell suspensions mixed with Matrigel. Human breast xenografts originated from primary breast carcinoma also frequently show the potential to metastasize spontaneously. In the present study, we generated a human breast carcinoma xenograft line (UISO-BCA-NMT-18) that shows 100% tumorigenicity and 80-100% lung metastasis when transplanted s.c. in athymic mice. We have studied in detail the characteristics of the xenograft and the patient's tumour from which the xenograft line originated. Both the xenograft and the patient's tumour showed intense staining for mutant p53 nuclear protein, and high expression of U-PA, PAI and u-PAR. In vivo growth of the xenograft is stimulated by exogenous supplementation of oestrogen. This xenograft is continuously growing in mice and has shown 80-100% metastasis for the last three successive in vivo passages. This well-characterized, oestrogen-responsive, metastatic breast carcinoma xenograft line will provide excellent research material for metastasis-related research. Images p596-a Figure 1 Figure 2 Figure 3 Figure 5 Figure 4 Figure 9 |
Databáze: | OpenAIRE |
Externí odkaz: |