Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation
| Autor: | Alex Vinicius Lopes Machado, Christiane Trevisan Slivinski, Ricardo Antonio Ayub, Jorge Iulek, Mareci Mendes de Almeida |
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| Rok vydání: | 2011 |
| Předmět: |
solid-state fermentation
Multidisciplinary Chromatography purification biology Ion exchange Starch lcsh:Biotechnology biochemical characterization Size-exclusion chromatography Aspergillus niger glucoamylase biology.organism_classification Hydrolysis chemistry.chemical_compound Solid-state fermentation chemistry lcsh:TP248.13-248.65 Fermentation Ammonium |
| Zdroj: | Brazilian Archives of Biology and Technology v.54 n.3 2011 Brazilian Archives of Biology and Technology Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR Brazilian Archives of Biology and Technology, Volume: 54, Issue: 3, Pages: 559-568, Published: JUN 2011 ResearcherID Brazilian Archives of Biology and Technology, Vol 54, Iss 3, Pp 559-568 (2011) |
| ISSN: | 1516-8913 |
| DOI: | 10.1590/s1516-89132011000300018 |
| Popis: | In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum temperature of 60 °C, with average activity 152.85 U mL-1. Thermal and pH stability assays with the crude extract showed that more than 60 % of the activity remained at pH 4.6 and 60 °C, even after an exposition to these conditions longer than 24 h. Yet, after purification, the enzyme was stable at these for at least 4 h, which indicated that its purification for use in starch saccharification was inadvisable. K M and Vmax were 0.34 mg mL-1 and 160.22 U mL-1, respectively. |
| Databáze: | OpenAIRE |
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