Effects of the Developmental Regulator BOLITA on the Plant Metabolome
| Autor: | David Díaz-Ramírez, José Juan Ordaz-Ortiz, Stefan de Folter, Ricardo A. Chávez Montes, Octavio Martínez de la Vega, Roberto Gamboa-Becerra, Nayelli Marsch-Martínez, Axel Tiessen-Favier, Irving Jair Garcia-Lopez, Robert Winkler, Hugo Gerardo Lazcano-Ramírez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Regulator Arabidopsis QH426-470 01 natural sciences Article 03 medical and health sciences Metabolomics Gene Expression Regulation Plant Gene expression Metabolome Genetics Arabidopsis thaliana glucosinolates Transcription factor global expression analysis Genetics (clinical) transcription factor metabolic fingerprinting biology Arabidopsis Proteins food and beverages Gene Expression Regulation Developmental phenylpropanoid pathway biology.organism_classification Cell biology Metabolic pathway 030104 developmental biology developmental regulation Transcriptome 010606 plant biology & botany Transcription Factors |
| Zdroj: | Genes Volume 12 Issue 7 Genes, Vol 12, Iss 995, p 995 (2021) |
| ISSN: | 2073-4425 |
| Popis: | Transcription factors are important regulators of gene expression. They can orchestrate the activation or repression of hundreds or thousands of genes and control diverse processes in a coordinated way. This work explores the effect of a master regulator of plant development, BOLITA (BOL), in plant metabolism, with a special focus on specialized metabolism. For this, we used an Arabidopsis thaliana line in which the transcription factor activity can be induced. Fingerprinting metabolomic analyses of whole plantlets were performed at different times after induction. After 96 h, all induced replicas clustered as a single group, in contrast with all controls which did not cluster. Metabolomic analyses of shoot and root tissues enabled the putative identification of differentially accumulated metabolites in each tissue. Finally, the analysis of global gene expression in induced vs. non-induced root samples, together with enrichment analyses, allowed the identification of enriched metabolic pathways among the differentially expressed genes and accumulated metabolites after the induction. We concluded that the induction of BOL activity can modify the Arabidopsis metabolome. Future work should investigate whether its action is direct or indirect, and the implications of the metabolic changes for development regulation and bioprospection. |
| Databáze: | OpenAIRE |
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