| Autor: |
Zheng, Zhijian, Wu, Ming, Li, Hongyan, Xu, Wenxia, Yang, Mengxiang, Pan, Kailing, Ni, Yuqi, Jiang, Ting, Zheng, Hongjuan, Jin, Xiayun, Zhang, Yanfei, Ding, Linchao, Fu, Jianfei |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.22617524.v1 |
| Popis: |
Additional file 1: Fig. S1. The coding potential of AC092894.1 and the gene location. (A)Coding potential score of AC092894.1measured based on CPC2.0. (B) Genomic structure of AC092894.1. Fig. S2. Knockdown of AC092894.1 inhibits the sensitivity of CRC cells to oxaliplatin. (A) Cell viability of HCT116 and HCT116-OxR cells treated with different concentrations of oxaliplatin for 48 h. (B) Knockdown efficiency of AC092894.1 detected by RT-qPCR. (C-H) The effect of oxaliplatin treatment after 48h was detected by CCK-8, colon formation, and apoptosis after knockdown of AC092894.1. (I) At the indicated concentrations, CCK-8 assayed the sensitivity of HCT116-OxR to 5-Fu. (J) At the indicated concentrations, CCK-8 assayed the sensitivity of HCT116-OxR to Irinotecan. (K) Overexpression of AC092894.1 on cells detected by CCK-8 after treatment with the indicated concentrations of 5-Fu for 48 h. (L) Overexpression of AC092894.1 on cells detected by CCK-8 after treatment with the indicated concentrations of Irinotecan for 48 h. Fig. S3. AC092894.1 does not promote chemo-sensitivity in CRC cells via the TNF signaling pathway.(A) Western blotting to assess TNF-α expression. (B)RT-qPCR assay of RASGRP3 expression in HCT116-OxR after overexpression of AC092894.1. Fig. S4. Effect of knockdown of RASGRP3 on p-p38 expression.Expression of p-p38 in cell lysates detected by western blotting; α-Tubulin was used as a control. Fig. S5. Expression of USP3 in patient tissues and detection of knockdown efficiency in cells. (A) Expression of USP3 in our cohort of CRC patients sensitive (n = 28) and resistant (n=22) to oxaliplatin treatment. (B) Western blotting to detect USP3 expression in cell lysates after silencing USP3 by siRNA in LoVo-OxR (AC092894.1) cells. Fig. S6. Effect of overexpression of AC092894.1 on RASGRP3 expression in mouse tumors. Representative IHC images showing RASGRP3 expression in tumors from each group of mice. Fig. S7. Effect of knockdown of RASGRP3 on AC092894.1/USP/AR.(A)RT-qPCR to detect the expression of AC092894.1.(B) Western blotting to assess AR expression. (C) Western blotting to assess USP3 expression. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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