Detection of respiratory syncytial virus antigen in nasal washings by Abbott TestPack enzyme immunoassay
| Autor: | C G Wren, B A Lauer, H B Masters, B J Bate |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Microbiology (medical)
Paramyxoviridae Antibodies Viral Immunofluorescence Binding Competitive Respirovirus Infections Sensitivity and Specificity Virus Immunoenzyme Techniques Antigen Culture Techniques Nasopharynx mental disorders medicine Animals Humans Child Antigens Viral Cytopathic effect chemistry.chemical_classification medicine.diagnostic_test biology Infant Abbott Diagnostics biology.organism_classification Virology Respiratory Syncytial Viruses Enzyme chemistry Immunoassay Rabbits Research Article |
| Zdroj: | Journal of Clinical Microbiology. 28:1395-1397 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.28.6.1395-1397.1990 |
| Popis: | We compared the new Abbott TestPack (TP) respiratory syncytial virus (RSV) enzyme immunoassay (EIA) with cell culture and two commercial RSV EIAs (from Abbott Diagnostics and Kallestad Laboratories) by using split samples of fresh nasal washings from children with suspected RSV disease. Two tubes of HEp-2 cells were inoculated and observed for cytopathic effect for 14 days, and isolates were confirmed by immunofluorescence. The TP EIA was performed by following the manufacturer's instructions. Specimens positive by TP EIA but negative by culture were examined in a competitive inhibition (blocking) assay using the TP EIA, and rabbit anti-RSV serum. Of 218 specimens, 93 were positive by culture, 105 were positive by TP EIA, 80 were positive by the Abbott Diagnostics EIA, and 87 were positive by the Kallestad Laboratories EIA. The sensitivity, specificity, positive predictive value, and negative predictive value of the TP EIA were 92, 86, 81, and 93%, respectively. Of 20 apparently false-positive TP EIAs, 10 of 14 that were positive when retested were neutralized in the blocking assay, indicating that they were truly positive. The recalculated sensitivity, specificity, positive predictive value, and negative predictive value of the TP EIA were 92, 91, 90, and 93%, respectively. We conclude that the TP EIA is easy to perform, rapid (less than 0.5 h), and accurate. |
| Databáze: | OpenAIRE |
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