Agentes osmóticos e temperatura na conservação in vitro de sempre-viva
| Autor: | Mara Márcia Sampaio Albuquerque, Moema Cortizo Bellintani, Sheila Vitória Resende, Alone Lima Brito, Bruno Freitas Matos Alvim, José Raniere Ferreira de Santana |
|---|---|
| Jazyk: | portugalština |
| Rok vydání: | 2011 |
| Předmět: |
food.ingredient
Sucrose General Veterinary Inoculation food and beverages Biology Horticulture chemistry.chemical_compound Tissue culture conservação ex situ food chemistry Shoot Botany cultura in vitro medicine Agar Animal Science and Zoology Sorbitol Mannitol crescimento mínimo Agronomy and Crop Science Syngonanthus mucugensis subsp. mucugensis Explant culture medicine.drug |
| Zdroj: | Ciência Rural v.41 n.8 2011 Ciência Rural Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
| Popis: | The in vitro conservation is an ex situ conservation strategy that ensures the maintenance of genetic and biological integrity of species. The present study evaluated the effects of, osmotic agents and different temperature regimes on the in vitro conservation of Syngonanthus mucugensis Giul. subsp. mucugensis. The shoots were inoculated into half salt strength Murashige and Skoog culture medium (MS ½) containing 7g L-1 of agar. The culture medium was supplemented with 60gL-1 sucrose and with the sucrose concentrations 15, 30 and 45g L-1 combined with 0 and 15g L-1 of sorbytol or mannitol. Two different temperatures were used in these experiments (18 and 25°C). The percentage of plant survival was evaluated monthly and at 180 days were analyzed length of shoot and root, the percentage of green leaves, the percentage of explants with shoots and number of shoots per explants and shoot length. The addition of osmotic agents resulted in decreased growth of the plants and therefore reduced their viability. The averages observed in the experiments undertaken at 18°C were significantly superior to those observed at 25°C for all of the variables analyzed. S. mucugensis subsp. mucugensis can be cultured at 18°C in MS½ culture medium containing 15g L-1 of sucrose, for up to 180 days, without subculturing. |
| Databáze: | OpenAIRE |
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