An uncoupling screen for autonomous embryo mutants in Arabidopsis thaliana

Autor:	Roger I. Pennell, Rod J. Scott, Uta Praekelt, Robert C. Day, Hong Pu, Nick Fenby
Rok vydání:	2010
Předmět:	Ovule Genetics education.field_of_study Mutant Population Arabidopsis food and beverages Embryo Cell Biology Plant Science Biology biology.organism_classification Endosperm Genetic Techniques Fertilization Mutation Pollen Arabidopsis thaliana education Gene Fertilisation Plant Proteins
Zdroj:	Sexual Plant Reproduction. 23:255-264
ISSN:	1432-2145 0934-0882
Popis:	Simple de novo screens in Arabidopsis thaliana have previously identified mutants that affect endosperm development but viable-embryo mutants have not been identified. Our strategy to identify autonomous embryo development was to uncouple embryo and endosperm fertilisation. This involved a male-sterile mutant population being crossed with a distinct pollen parent--the pollen was needed to initiate endosperm development and because it was distinct, the maternal progeny could be selected from the hybrid population. This process was refined over three stages, resulting in a viable approach to screen for autonomous embryo mutants. From 8,000 screened plants, a mutation was isolated in which the integument cells extended from the ovule and proliferated into a second complete twinned ovule. Some embryos from the mutant were normal but others developed fused cotyledons. In addition, a proportion of the progeny lacked paternal genes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53ebb577f062394737efd64595e64293 https://doi.org/10.1007/s00497-010-0142-z Zobrazit plný text záznamu Plný text ve formátu PDF