Analysis of the phenotypes exhibited by rudimentary-like mutants of Drosophila melanogaster
| Autor: | John Rawls, Timothy W Conner Ii |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
Orotic acid Carboxy-Lyases Orotate Phosphoribosyltransferase Orotidine-5'-Phosphate Decarboxylase Mutant Biology Biochemistry Genetics medicine Animals Pentosyltransferases Molecular Biology Ecology Evolution Behavior and Systematics Orotic Acid chemistry.chemical_classification Phenocopy Eye Color fungi General Medicine biology.organism_classification Phenotype Drosophila melanogaster Pyrimidines Enzyme chemistry Mutation Pyrimidine metabolism Epistasis Female medicine.drug |
| Zdroj: | Biochemical Genetics. 20:607-619 |
| ISSN: | 1573-4927 0006-2928 |
| DOI: | 10.1007/bf00483960 |
| Popis: | Flies mutant for one or both of the last two enzymes of de novo pyrimidine biosynthesis express a number of phenotypes that are also expressed by mutants of the first four pathway enzymes (r and Dhod-null mutants). However, r-1 flies also express two phenotypes, mottled eyes and poor viability, that are not usually expressed by r and Dhod-null flies. Chemical determinations show that orotic acid, a substrate for the fifth pathway enzyme, accumulates in r-1 individuals but not in r and wild-type individuals. Moreover, flies simultaneously mutant for r and r-1 do not express the mottled-eye phenotype, showing that r is epistatic to r-1 for this r-1-specific phenotype. When genotypically wild-type flies are cultured on a medium containing 6-azauracil, the base of a potent inhibitor of the last enzyme of de novo pyrimidine biosynthesis, phenocopies are obtained that include the mottled-eye as well as the wing phenotypes of r-1 flies. These results support hypotheses that the phenotypes common to r, Dhod-null, and r-1 flies are consequences of uridylic acid deficiency, whereas the r-1-specific phenotypes result from orotic acid accumulation in flies lacking either or both of the last two enzymes of de novo pyrimidine biosynthesis. |
| Databáze: | OpenAIRE |
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